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Down‐regulation of miRNA‐27b‐3p suppresses keratinocytes apoptosis in oral lichen planus
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Oral lichen planus (OLP) is considered a precancerous lesion with no known cure. Recent studies reported that abnormal regulation of apoptosis was involved in the pathogenesis of OLP. Next generation sequencing was used to screen the candidate microRNAs and genes in biopsies from patients with OLP and healthy mucosa. Human oral keratinocytes were transfected into the related oligonucleotides of miR‐27b‐3p/cyclophilin D and their control groups. Apoptosis was detected by TdT‐mediated dUTP nick end labelling and flow cytometry. The levels of mRNA and protein were detected by quantitative PCR, Western blots, and enzyme‐linked immunosorbent assays, respectively. Luciferase assays were performed to detect the luciferase activities of miR‐27b‐3p and cyclophilin D. Here, we showed that basal epithelium apoptosis was reduced and the miR‐27b‐3p levels were decreased in clinical OLP samples. We also found that down‐regulation of miR‐27b‐3p inhibited epithelial keratinocyte apoptosis by up‐regulating cyclophilin D expression. Moreover, cyclophilin D increased the protein stability of Bcl2 through direct binding, and Bcl2 suppressed caspase9/3 activation and cytochrome C release. Taken together, these data showed that miR‐27b‐3p regulated keratinocyte apoptosis through cyclophilin D/Bcl2 signalling, suggesting the miR‐27b‐3p regulated the pathogenesis of OLP.
- Subjects :
- Adult
Keratinocytes
Male
0301 basic medicine
miR‐27b‐3p
Flow cytometry
Cyclophilins
Young Adult
03 medical and health sciences
0302 clinical medicine
stomatognathic system
microRNA
medicine
Humans
Luciferase
Cyclophilin
Aged
Cell Proliferation
medicine.diagnostic_test
Chemistry
apoptosis
Original Articles
Cell Biology
Middle Aged
medicine.disease
Molecular biology
Blot
MicroRNAs
stomatognathic diseases
030104 developmental biology
Real-time polymerase chain reaction
Gene Expression Regulation
Apoptosis
Case-Control Studies
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Molecular Medicine
Female
Mouth Neoplasms
Original Article
Oral lichen planus
Oral Lichen Planus
epithelium
cyclophilin D
Lichen Planus, Oral
Signal Transduction
Subjects
Details
- ISSN :
- 15824934 and 15821838
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....80c0870035fdd3e959027cd81ebee3bd