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Effect of vasoactive intestinal peptide on serotonin release in the suprachiasmatic area of the rat. Modulation by oestradiol
- Source :
- Peptides. 5:313-317
- Publication Year :
- 1984
- Publisher :
- Elsevier BV, 1984.
-
Abstract
- The effect of vasoactive intestinal peptide (VIP) on spontaneous and induced release of newly synthesized 5-hydroxytryptamine (5-HT) was studied in the suprachiasmatic area (SCA) using a superfusion system. To test the possible modualtion by E 2 on the interaction VIP-5-HT, the experiments were conducted on male, ovariectomized (OVX) and ovariectomized oestradiol implanted rats (OVX-E 2 ). VIP (10 −7 M) infused for 15 min caused an increase of 5-HT release from SCA of male and OVX. The positive effect of VIP on 5-HT release results partially from an inhibition of the reuptake of 5-HT: in male and OVX SCA, VIP inhibited the 3 H-5-HT uptake by 40 to 50%. The infusion of VIP before a pulse of K + (10-20-30-56 mM) leads to a potentialisation of the evoked release suggesting that VIP sensitized the presynaptic membrane to the process linking depolarization and release. When SCA taken from OVX-E 2 were exposed to VIP, 5-HT uptake and consequently 5-HT release were unchanged. The present results suggest that the metabolism of 5-HT in the SCA is influenced by VIP and that this regulation may be modulated by E 2 . This interaction between E 2 , VIP and 5-HT at the SCA level may be involved in the regulation of phasic LH and prolactin surge.
- Subjects :
- Male
Serotonin
medicine.medical_specialty
Physiology
Vasoactive intestinal peptide
In Vitro Techniques
Biology
Biochemistry
Reuptake
Cellular and Molecular Neuroscience
Endocrinology
Internal medicine
medicine
Animals
Castration
5-HT receptor
Estradiol
Suprachiasmatic nucleus
Ovary
Rats, Inbred Strains
Depolarization
Metabolism
Prolactin
Rats
Perfusion
Ovariectomized rat
Female
Suprachiasmatic Nucleus
hormones, hormone substitutes, and hormone antagonists
Vasoactive Intestinal Peptide
Subjects
Details
- ISSN :
- 01969781
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Peptides
- Accession number :
- edsair.doi.dedup.....80a37eff0a4f7f3ab3e88cc364dab70c