Back to Search
Start Over
One-step and DNA amplification-free detection of Listeria monocytogenes in ham samples: Combining magnetic relaxation switching and DNA hybridization reaction
- Source :
- Food chemistry. 338
- Publication Year :
- 2020
-
Abstract
- Early screening of L. monocytogenes in ready-to-eat food can prevent and control its harmful effects. In this study, we propose a highly sensitive magnetic DNA sensor based on nucleic acid hybridization reaction and magnetic signal readout. We design the L. monocytogenes specific probe1 and probe2 and label them on the 30 and 250 nm magnetic nanoparticles, respectively. The hybridization reaction between the magnetic probes and DNA of L. monocytogenes could form a sandwich nanocomplex. After magnetic separation, the unbound MNP30–probe2 can act as the transverse relaxation time (T2) signal readout probe. This assay allows the one-step detection of L. monocytogenes as low as 50 CFU/mL within 2 h without DNA amplification, and the average recovery in the spiked ham sausage samples can reach 92.6%. This system integrates the high sensitivity of magnetic sensing and high efficiency of hybridization reaction, providing a promising detection platform for pathogens.
- Subjects :
- DNA, Bacterial
Magnetic Resonance Spectroscopy
Magnetic separation
One-Step
medicine.disease_cause
01 natural sciences
Analytical Chemistry
Nucleic acid thermodynamics
chemistry.chemical_compound
0404 agricultural biotechnology
Listeria monocytogenes
medicine
Chromatography
Chemistry
DNA–DNA hybridization
Magnetic Phenomena
010401 analytical chemistry
Nucleic Acid Hybridization
04 agricultural and veterinary sciences
General Medicine
equipment and supplies
Dna amplification
040401 food science
0104 chemical sciences
Meat Products
Food Microbiology
Magnetic nanoparticles
Nanoparticles
DNA Probes
human activities
Nucleic Acid Amplification Techniques
DNA
Food Science
Subjects
Details
- ISSN :
- 18737072
- Volume :
- 338
- Database :
- OpenAIRE
- Journal :
- Food chemistry
- Accession number :
- edsair.doi.dedup.....808fb30da0ddac7fa707ac6ca9b83f7c