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Nutrient restriction of glucose or serum results in similar proteomic expression changes in 3D colon cancer cell cultures
- Source :
- Nutrition Research. 36:1068-1080
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Nutrient restriction, also known as caloric restriction, has been extensively examined for its positive impact on lifespan, immune system boost, and aging. In addition, nutrient restriction is implicated in decreasing cancer initiation and progression. Given the phenotypic changes associated with nutrient restriction, we hypothesized significant protein expression alterations must be associated with caloric restriction. To compare the molecular and phenotypic changes caused by glucose restriction and fetal bovine serum (FBS) restriction there is need for an efficient model system. We establish three dimensional cell culture models, known as spheroids, in the HCT 116 colorectal cancer cell line as a high throughput model for studying the proteomic changes associated with nutrient restriction. Flow cytometry was used to assess apoptosis and autophagy levels in the spheroids under nutrient restriction. Isobaric tags for relative and absolute quantification (iTRAQ) and liquid chromatography tandem mass spectrometry were used to determine differential protein abundances between the nutrient restriction conditions. We identified specific proteins that have implications in cancer progression and metastasis that are differentially regulated by restriction of either glucose or serum. These proteins include the up regulation of sirtuin 1 (SIRT1) and protein inhibitor of activated STAT 1 (PIAS1) and down regulation of multi-drug resistance protein (MRP1) and Zinc finger and BTB domain-containing protein 7A (ZBTB7). The results indicate nutrient restriction causes lower apoptotic and higher autophagy rates in HCT 116 spheroids. In addition, proteins shown to be differentially regulated by both glucose and serum restriction were similarly regulated.
- Subjects :
- Serum
0301 basic medicine
Endocrinology, Diabetes and Metabolism
Quantitative proteomics
Down-Regulation
Apoptosis
Models, Biological
Article
Flow cytometry
03 medical and health sciences
Endocrinology
Sirtuin 1
Downregulation and upregulation
Tandem Mass Spectrometry
Zbtb7
Cell Line, Tumor
Autophagy
medicine
Animals
Humans
Protein inhibitor of activated STAT
Caloric Restriction
Nutrition and Dietetics
medicine.diagnostic_test
biology
Proteins
Flow Cytometry
Protein Inhibitors of Activated STAT
Up-Regulation
DNA-Binding Proteins
Glucose
030104 developmental biology
Biochemistry
Small Ubiquitin-Related Modifier Proteins
biology.protein
Cattle
Multidrug Resistance-Associated Proteins
Colorectal Neoplasms
Fetal bovine serum
Chromatography, Liquid
Transcription Factors
Subjects
Details
- ISSN :
- 02715317
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Nutrition Research
- Accession number :
- edsair.doi.dedup.....80861241efdfb6d145f1568532727b61