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A Non-radioassay of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase by Anion-Exchange Chromatography
- Source :
- Bioscience, biotechnology, and biochemistry. 62(3)
- Publication Year :
- 2016
-
Abstract
- A non-radioisotopic assay method for ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is described. 3-Phosphoglycerate produced by the RuBisCO reaction was measured using an anion-exchange chromatography. The reaction course of spinach RuBisCO measured by this method was completely the same as that measured with (14)CO2.
- Subjects :
- inorganic chemicals
Oxygenase
Ion chromatography
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
chemistry.chemical_compound
Molecular Biology
chemistry.chemical_classification
Chromatography
Ribulose 1,5-bisphosphate
biology
Chemistry
Ribulose
fungi
Organic Chemistry
RuBisCO
food and beverages
General Medicine
biology.organism_classification
Pyruvate carboxylase
Enzyme
biology.protein
Spinach
Biotechnology
Subjects
Details
- ISSN :
- 09168451
- Volume :
- 62
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Accession number :
- edsair.doi.dedup.....8084dcab59594c3f142f5bf04822fa93