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High Affinity Antibodies against Influenza Characterize the Plasmablast Response in SLE Patients After Vaccination
- Source :
- PLoS ONE, Vol 10, Iss 5, p e0125618 (2015)
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- Breakdown of B cell tolerance is a cardinal feature of systemic lupus erythematosus (SLE). Increased numbers of autoreactive mature naïve B cells have been described in SLE patients and autoantibodies have been shown to arise from autoreactive and non-autoreactive precursors. How these defects, in the regulation of B cell tolerance and selection, influence germinal center (GC) reactions that are directed towards foreign antigens has yet to be investigated. Here, we examined the characteristics of post-GC foreign antigen-specific B cells from SLE patients and healthy controls by analyzing monoclonal antibodies generated from plasmablasts induced specifically by influenza vaccination. We report that many of the SLE patients had anti-influenza antibodies with higher binding affinity and neutralization capacity than those from controls. Although overall frequencies of autoreactivity in the influenza-specific plasmablasts were similar for SLE patients and controls, the variable gene repertoire of influenza-specific plasmablasts from SLE patients was altered, with increased usage of JH6 and long heavy chain CDR3 segments. We found that high affinity anti-influenza antibodies generally characterize the plasmablast responses of SLE patients with low levels of autoreactivity; however, certain exceptions were noted. The high-avidity antibody responses in SLE patients may also be correlated with cytokines that are abnormally expressed in lupus. These findings provide insights into the effects of dysregulated immunity on the quality of antibody responses following influenza vaccination and further our understanding of the underlying abnormalities of lupus.
- Subjects :
- Naive B cell
Antibody Affinity
lcsh:Medicine
03 medical and health sciences
0302 clinical medicine
Antigen
immune system diseases
medicine
Humans
Lupus Erythematosus, Systemic
skin and connective tissue diseases
lcsh:Science
B cell
030304 developmental biology
030203 arthritis & rheumatology
0303 health sciences
Multidisciplinary
Lupus erythematosus
Systemic lupus erythematosus
biology
lcsh:R
Autoantibody
Antibodies, Monoclonal
Germinal center
Orthomyxoviridae
medicine.disease
Virology
3. Good health
medicine.anatomical_structure
Influenza Vaccines
Case-Control Studies
Antibody Formation
Immunology
biology.protein
lcsh:Q
Antibody
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8070eac38793bcf200eb4155569c3d8d