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Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression
- Source :
- BMC Veterinary Research, Vol 16, Iss 1, Pp 1-11 (2020), BMC Veterinary Research
- Publication Year :
- 2020
- Publisher :
- BMC, 2020.
-
Abstract
- Background Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR. Results The histopathological assessment showed noticeable signs of skin healing in TCG compared to CG. Immunohistochemistry results revealed remarkable enhancement in the expression levels of VEGF and TGF-β1 in TCG. Furthermore, TCG exhibited marked upregulation in the VEGF, bFGF, and α-SMA genes expression. These findings suggested that the topical application of Nile tilapia collagen extract can promote the cutaneous wound healing process in rats, which could be attributed to its stimulating effect on recruiting and activating macrophages to produce chemotactic growth factors, fibroblast proliferation, and angiogenesis. Conclusions The collagen extract could, therefore, be a potential biomaterial for cutaneous wound healing therapeutics.
- Subjects :
- Male
Angiogenesis
Tilapia collagen
Basic fibroblast growth factor
Histopathology
Wound healing
Andrology
030207 dermatology & venereal diseases
03 medical and health sciences
chemistry.chemical_compound
Nile tilapia
0302 clinical medicine
medicine
Animals
Rats, Wistar
Fibroblast
Skin
030304 developmental biology
Skin repair
0303 health sciences
lcsh:Veterinary medicine
General Veterinary
biology
Vascular Endothelial Growth Factors
Cichlids
General Medicine
α-SMA
biology.organism_classification
VEGF
Actins
Vascular endothelial growth factor
medicine.anatomical_structure
chemistry
bFGF
TGF-ß
lcsh:SF600-1100
Fibroblast Growth Factor 2
Collagen
Gene expression
Research Article
IHC
Transforming growth factor
Subjects
Details
- Language :
- English
- ISSN :
- 17466148
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....8060c0fce63a237f01bc56e7594d67c2