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Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 7993, p 7993 (2021), International journal of molecular sciences, International Journal of Molecular Sciences, Volume 22, Issue 15
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Salt and osmotic stress are the main abiotic stress factors affecting plant root growth and architecture. We investigated the effect of salt (100 mM NaCl) and osmotic (200 mM mannitol) stress on the auxin metabolome by UHPLC-MS/MS, auxin distribution by confocal microscopy, and transcript levels of selected genes by qRT-PCR in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and DR5rev::GFP (DR5) line. During long-term stress (13 days), a stability of the auxin metabolome and a tendency to increase indole-3-acetic acid (IAA) were observed, especially during salt stress. Short-term stress (3 h) caused significant changes in the auxin metabolome, especially NaCl treatment resulted in a significant reduction of IAA. The data derived from auxin profiling were consistent with gene expressions showing the most striking changes in the transcripts of YUC, GH3, and UGT transcripts, suggesting disruption of auxin biosynthesis, but especially in the processes of amide and ester conjugation. These data were consistent with the auxin distribution observed in the DR5 line. Moreover, NaCl treatment caused a redistribution of auxin signals from the quiescent center and the inner layers of the root cap to the epidermal and cortical cells of the root elongation zone. The distribution of PIN proteins was also disrupted by salt stress<br />in particular, PIN2 was suppressed, even after 5 min of treatment. Based on our results, the DR5 line was more sensitive to the applied stresses than Col-0, although both lines showed similar trends in root morphology, as well as transcriptome and metabolome parameters under stress conditions.
- Subjects :
- 0106 biological sciences
0301 basic medicine
abiotic stress
Osmotic shock
root growth
QH301-705.5
Arabidopsis thaliana
auxin distribution
auxin metabolome
auxin transcriptome
01 natural sciences
Catalysis
Inorganic Chemistry
03 medical and health sciences
Auxin
Metabolome
medicine
heterocyclic compounds
Physical and Theoretical Chemistry
PIN proteins
Biology (General)
Molecular Biology
Root cap
Biology
QD1-999
Spectroscopy
chemistry.chemical_classification
biology
Chemistry
Abiotic stress
Organic Chemistry
fungi
Biochemistry and Molecular Biology
food and beverages
General Medicine
biology.organism_classification
Computer Science Applications
Cell biology
030104 developmental biology
Mannitol
010606 plant biology & botany
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 7993
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....8058b6365983513ed85f7f75b538e8b4