Fluorescence of native and carotenoid-depleted LH2 from Chromatium minutissimum, originating from simultaneous two-photon absorption in the spectral range of the presumed (optically ‘dark’) S1 state of carotenoids

Authors :: Andrei P. Razjivin
Maria Krikunova
Andrey A. Moskalenko
Dieter Leupold
Hugo Scheer
Heiko Lokstein
B. Voigt
M Rini
A Kummrow
Source :: FEBS Letters. (1-3):227-229
Publisher :: Federation of European Biochemical Societies. Published by Elsevier B.V.
Abstract: Native and carotenoid-depleted peripheral purple bacterial light-harvesting complex (LH2) were investigated by simultaneous two-photon excited (between 1300–1500 nm) fluorescence (TPF). TPF results from direct bacteriochlorophyll excitation in both samples. The spectral position of the 2A g − state of rhodopsin is indicated by a diminuition of the bacteriochlorophyll TPF in native LH2. In conclusion, comparison to carotenoid-depleted samples is a conditio sine qua non for unambiguous interpretation of similar experiments.

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