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Endothelium-Dependent and -Independent Vasodilator Effects of Dimethyl Sulfoxide in Rat Aorta
- Source :
- Pharmacology. 97(3-4)
- Publication Year :
- 2015
-
Abstract
- This study examined the mechanism of vasorelaxation induced by dimethyl sulfoxide (DMSO) in endothelium-intact and -denuded rat aorta. DMSO (0.1-3%) inhibited phenylephrine (PE, 1 μmol/l)-induced contraction in a dose-dependent manner. However, this relaxation was lower in the absence of the endothelium. Increase in DMSO-induced relaxation in the presence of the endothelium was attenuated by preincubation in L-NG-nitroarginine methyl ester (L-NAME, 100 μmol/l) and by the removal of the endothelium. In the aorta with endothelium, DMSO (3%) and CCh (3 μmol/l) increased cGMP contents, significantly and L-NAME (100 μmol/l) inhibited the DMSO-induced increases of cGMP. In fura 2-loaded endothelium-denuded aorta, cumulative application of DMSO (1-3%) inhibited PE-induced muscle tension; however, this application did not affect the [Ca2+]i level. In PE-precontracted endothelium-denuded aorta, relaxation responses to fasudil were significantly less in the presence of DMSO compared to the control. These results suggest that DMSO causes relaxation by increasing the cGMP content in correlation with the release of NO from endothelial cells and by decreasing the Ca2+ sensitivity of contractile elements partly via inhibiting Rho-kinase in rat aorta.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Contraction (grammar)
Endothelium
Vasodilator Agents
Vasodilation
Aorta, Thoracic
03 medical and health sciences
chemistry.chemical_compound
Phenylephrine
Internal medicine
medicine.artery
Muscle tension
medicine
Animals
Dimethyl Sulfoxide
Rats, Wistar
Cyclic GMP
Pharmacology
Aorta
Dimethyl sulfoxide
business.industry
Fasudil
General Medicine
Surgery
Rats
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
cardiovascular system
Calcium
Endothelium, Vascular
business
medicine.drug
Muscle Contraction
Subjects
Details
- ISSN :
- 14230313
- Volume :
- 97
- Issue :
- 3-4
- Database :
- OpenAIRE
- Journal :
- Pharmacology
- Accession number :
- edsair.doi.dedup.....8027610045f36b3d1ffd979cd6b909eb