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Metabarcoding analysis of the stomach contents of the Antarctic Toothfish (Dissostichus mawsoni) collected in the Antarctic Ocean
- Source :
- PeerJ, PeerJ, Vol 5, p e3977 (2017)
- Publication Year :
- 2017
- Publisher :
- PeerJ Inc., 2017.
-
Abstract
- Stomach contents of the Antarctic toothfish, Dissostichus mawsoni, collected from subareas 58.4 and 88.3, were analyzed using next generation sequencing (NGS) technology. After processing the raw reads generated by the MiSeq platform, a total of 131,233 contigs (130 operational taxonomic units [OTUs]) were obtained from 163 individuals in subarea 58.4, and 75,961 contigs (105 OTUs) from 164 fish in subarea 88.3. At 98% sequence identity, species names were assigned to most OTUs in this study, indicating the quality of the DNA barcode database for the Antarctic Ocean was sufficient for molecular analysis, especially for fish species. A total of 19 species was identified from the stomach of D. mawsoni in this study, which included 14 fish species and five mollusks. More than 90% of contigs belonged to fish species, supporting the postulate that the major prey of D. mawsoni are fish. Two fish species, Macrourus whitsoni and Chionobathyscus dewitti, were the most important prey items (a finding similar to that of previous studies). We also obtained genotypes of prey items by NGS analysis, identifying an additional 17 representative haplotypes in this study. Comparison with three previous morphological studies and the NGS-based molecular identification in this study extended our knowledge regarding the prey of D. mawsoni, which previously was not possible. These results suggested that NGS-based diet studies are possible, if several current technical limitations, including the quality of the barcode database or the development of precise molecular quantification techniques to link them with morphological values, are overcome. To achieve this, additional studies should be conducted on various marine organisms.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Dissostichus
Zoology
lcsh:Medicine
Marine Biology
Stomach contents
Barcode
010603 evolutionary biology
01 natural sciences
DNA barcoding
General Biochemistry, Genetics and Molecular Biology
DNA sequencing
law.invention
Predation
03 medical and health sciences
Chionobathyscus dewitti
law
Foodweb
Antarctic toothfish
biology
Ecology
General Neuroscience
lcsh:R
General Medicine
biology.organism_classification
Sequence identity
030104 developmental biology
Metabarcoding
Next-generation sequencing
Antarctica
General Agricultural and Biological Sciences
Subjects
Details
- Language :
- English
- ISSN :
- 21678359
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- PeerJ
- Accession number :
- edsair.doi.dedup.....7fb7d9d4e7c1614d3f98ea753c935fbd