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Avian lungs: A novel scaffold for lung bioengineering
- Source :
- PLoS ONE, Vol 13, Iss 6, p e0198956 (2018), PLoS ONE
- Publication Year :
- 2018
- Publisher :
- Public Library of Science (PLoS), 2018.
-
Abstract
- Allogeneic lung transplant is limited both by the shortage of available donor lungs and by the lack of suitable long-term lung assist devices to bridge patients to lung transplantation. Avian lungs have different structure and mechanics resulting in more efficient gas exchange than mammalian lungs. Decellularized avian lungs, recellularized with human lung cells, could therefore provide a powerful novel gas exchange unit for potential use in pulmonary therapeutics. To initially assess this in both small and large avian lung models, chicken (Gallus gallus domesticus) and emu (Dromaius novaehollandiae) lungs were decellularized using modifications of a detergent-based protocol, previously utilized with mammalian lungs. Light and electron microscopy, vascular and airway resistance, quantitation and gel analyses of residual DNA, and immunohistochemical and mass spectrometric analyses of remaining extracellular matrix (ECM) proteins demonstrated maintenance of lung structure, minimal residual DNA, and retention of major ECM proteins in the decellularized scaffolds. Seeding with human bronchial epithelial cells, human pulmonary vascular endothelial cells, human mesenchymal stromal cells, and human lung fibroblasts demonstrated initial cell attachment on decellularized avian lungs and growth over a 7-day period. These initial studies demonstrate that decellularized avian lungs may be a feasible approach for generating functional lung tissue for clinical therapeutics.
- Subjects :
- 0301 basic medicine
Pathology
medicine.medical_treatment
Respiratory System
lcsh:Medicine
Apoptosis
Biochemistry
Poultry
Diagnostic Radiology
Extracellular matrix
Medicine and Health Sciences
Gamefowl
Respiratory System Procedures
lcsh:Science
Lung
Staining
Extracellular Matrix Proteins
Multidisciplinary
Decellularization
Tissue Scaffolds
Radiology and Imaging
Eukaryota
Cell Staining
respiratory system
Pulmonary Imaging
Extracellular Matrix
medicine.anatomical_structure
Vertebrates
Immunohistochemistry
Anatomy
Research Article
Lung Transplantation
medicine.medical_specialty
Imaging Techniques
Bioengineering
Surgical and Invasive Medical Procedures
Biology
Research and Analysis Methods
Birds
03 medical and health sciences
Diagnostic Medicine
medicine
Lung transplantation
Animals
Humans
Cell Proliferation
Transplantation
Dromaiidae
Cell growth
Mesenchymal stem cell
lcsh:R
Organisms
Biology and Life Sciences
Proteins
Organ Transplantation
respiratory tract diseases
030104 developmental biology
Fowl
Specimen Preparation and Treatment
Amniotes
lcsh:Q
Lungs
Chickens
Collagens
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....7fb7479c84ebafa651a96bb5eae70e8c