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Impact of the Type I Interferon Receptor on the Global Gene Expression Program During the Course of Dendritic Cell Maturation Induced by Polyinosinic Polycytidylic Acid
- Publication Year :
- 2016
- Publisher :
- Mary Ann Liebert, Inc., 2016.
-
Abstract
- Dendritic cell (DC) maturation involves widespread changes in cellular function and gene expression. The regulatory role of IFNAR in the program of DC maturation remains incompletely defined. Thus, the time evolution impact of IFNAR on this process was evaluated. Changes in DC phenotype, function, and gene expression induced by poly I:C were measured in wild-type and IFNAR(-/-) DC at 9 time points over 24 h. Temporal gene expression profiles were filtered on consistency and response magnitude across replicates. The number of genes whose expression was altered by poly I:C treatment was greatly reduced in IFNAR(-/-) DC, including the majority of the downregulated gene expression program previously observed in wild-type (WT) DC. Furthermore, the number of genes upregulated was almost equal between WT and IFNAR(-/-) DC, yet the identities of those genes were distinct. Integrating these data with protein-protein interaction data revealed several novel subnetworks active during maturation, including nucleotide synthesis, metabolism, and repair. A subnetwork associated with redox activity was uniquely identified in IFNAR(-/-) DC. Overall, temporal gene expression and network analyses identified many genes regulated by the type I interferon response and revealed previously unidentified aspects of the DC maturation process.
- Subjects :
- 0301 basic medicine
Cellular differentiation
T-Lymphocytes
Immunology
Receptor, Interferon alpha-beta
Biology
Lymphocyte Activation
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Virology
Gene expression
Animals
Gene
Genetics
Regulation of gene expression
Mice, Knockout
Gene Expression Profiling
Computational Biology
Research Reports
Cell Differentiation
Molecular Sequence Annotation
Cell Biology
Dendritic cell
Dendritic Cells
Phenotype
Cell biology
Gene expression profiling
030104 developmental biology
Poly I-C
chemistry
Gene Expression Regulation
Polyinosinic:polycytidylic acid
Cytokines
Female
Transcriptome
030215 immunology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7f9be1f2b371dfd47459a198b07a7cd5