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Autoantibody-producing RP105- B cells, from patients with systemic lupus erythematosus, showed more preferential expression of BCMA compared with BAFF-R than normal subjects
- Source :
- Rheumatology. 49:662-670
- Publication Year :
- 2010
- Publisher :
- Oxford University Press (OUP), 2010.
-
Abstract
- OBJECTIVE B cells lacking RP105 produce autoantibodies in patients with SLE. Expression of B-cell activating factor (BAFF) binding receptors (BBRs) and survival of RP105(-) B cells from SLE patients were examined. METHODS Detection of difference of gene expression between RP105(-) and RP105(+) B cells was done by DNA microarrays. Surface expression was confirmed by flow cytometry. The contribution of BAFF, a proliferation-inducing ligand (APRIL) and monomers/trimers of sCD40L to survival of RP105(-) and RP105(+) B cells was examined. RESULTS Gene expression of B-cell maturation antigen (BCMA) was different among BBRs in RP105(-) and RP105(+) B cells in SLE. Preferential expression of BCMA on RP105(-) B cells was confirmed compared with RP105(+) B cells by flow cytometry, although BAFF receptor (BAFF-R) expression on RP105(-) B cells was significantly lower. Additionally, relative ratios of BCMA/BAFF-R expression on RP105(-) B cells were increased significantly in SLE patients compared with normal subjects. Stimulation by sCD40L decreased the number of surviving RP105(-) and RP105(+) B cells in vitro. RP105(+) B cells were not rescued from sCD40L-induced cell death by BAFF and/or APRIL. In contrast, either BAFF or APRIL maintained the survival of RP105(-) B cells due to avoidance of cell death. Activated RP105(-) B cells reduced BAFF-R and increased BCMA levels. CONCLUSIONS RP105(-) B cells from SLE patients showed more preferential expression of BCMA compared with BAFF-R than normal subjects, and were possibly regulated by BAFF/APRIL. Our results provide a new insight of BCMA and their ligands in B cells from SLE patients.
- Subjects :
- Adult
Male
medicine.medical_specialty
Adolescent
medicine.disease_cause
Polymerase Chain Reaction
Autoimmunity
Flow cytometry
Young Adult
Rheumatology
Antigen
immune system diseases
Internal medicine
medicine
Humans
Lupus Erythematosus, Systemic
Pharmacology (medical)
B-Cell Maturation Antigen
skin and connective tissue diseases
BAFF receptor
B-cell activating factor
Aged
Autoantibodies
CD40
biology
medicine.diagnostic_test
business.industry
Autoantibody
Middle Aged
Flow Cytometry
Molecular biology
Endocrinology
Case-Control Studies
biology.protein
Female
business
B-Cell Activation Factor Receptor
Subjects
Details
- ISSN :
- 14620332 and 14620324
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Rheumatology
- Accession number :
- edsair.doi.dedup.....7f95eed903eb0a895f9680b548477271