Retardation and Regression of Lymphosarcoma in C3H Mice Treated with Alien Mouse Spleen and A-Methopterin

SummaryUnder certain conditions C3H mice implanted with lymphosarcoma 6-C3HED were protected either by injection of ZBC spleen tissue alone or by A-methopterin treatment alone. A more successful treatment consisted of a single subcutaneous injection of ZBC spleen tissue with daily doses of A-methopterin. Mice which had received tumor implantations 24, 48 or 96 hours preceding therapy were treated with this combination. Some were not protected, but in others tumors either failed to become palpable, or having become palpable appeared to have regressed completely.