Additional file 1: of Cytotoxicity and cell cycle arrest induced by andrographolide lead to programmed cell death of MDA-MB-231 breast cancer cell line

Andrographolide-induced externalization of phosphatidyl serine and apoptosis in MCF-7 cells. Figure S1. Effect of andrographolide treatment on apoptosis in MCF-7 cells. Cells were treated with IC50 concentration of andrographolide for 48Â h, double stained with annexin V-FITC/PI and analyzed in a FACSVerseâ ˘ (Becton Dickinson, USA) flow cytometer. The percentage Annexin V-positive population refers apoptosis induction (region 2 and 4). Data are representative of three independent experiments. (PDF 88Â kb)