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Phylogenetic and phenotypic characterization of Fusarium oxysporum f. sp. niveum isolates from Florida-grown watermelon
- Source :
- PLoS ONE, PLoS ONE, Vol 16, Iss 3, p e0248364 (2021)
- Publication Year :
- 2020
-
Abstract
- Fusarium wilt of watermelon (Citrullus lanatus) caused by Fusarium oxysporum f. sp. niveum (Fon), has become an increasing concern of farmers in the southeastern USA, especially in Florida. Management of this disease, most often through the use of resistant cultivars and crop rotation, requires an accurate understanding of an area’s pathogen population structure and phenotypic characteristics. This study improved the understanding of the state’s pathogen population by completing multilocus sequence analysis (MLSA) of two housekeeping genes (BT and TEF) and two loci (ITS and IGS), aggressiveness and race-determining bioassays on 72 isolates collected between 2011 and 2015 from major watermelon production areas in North, Central, and South Florida. Multilocus sequence analysis (MLSA) failed to group race 3 isolates into a single large clade; moreover, clade membership was not apparently correlated with aggressiveness (which varied both within and between clades), and only slightly with sampling location. The failure of multilocus sequence analysis using four highly conserved housekeeping genes and loci to clearly group and delineate known Fon races provides justification for future whole genome sequencing efforts whose more robust genomic comparisons will provide higher resolution of intra-species genetic distinctions. Consequently, these results suggest that identification of Fon isolates by race determination alone may fail to detect economically important phenotypic characteristics such as aggressiveness leading to inaccurate risk assessment.
- Subjects :
- Heredity
Pathology and Laboratory Medicine
Geographical locations
Fusarium
Medicine and Health Sciences
Clade
Data Management
Genetics
Fungal Pathogens
education.field_of_study
Multidisciplinary
biology
Phylogenetic tree
Geography
food and beverages
Phylogenetic Analysis
Fusarium wilt
Housekeeping gene
Phylogenetics
Phylogeography
Genetic Mapping
Biogeography
Medical Microbiology
Florida
Medicine
Pathogens
Research Article
Computer and Information Sciences
Citrullus lanatus
Sequence analysis
Science
Population
Mycology
Microbiology
Citrullus
Fusarium oxysporum
Animals
Evolutionary Systematics
Fusarium Oxysporum
education
Microbial Pathogens
Plant Diseases
Taxonomy
Evolutionary Biology
Population Biology
Ecology and Environmental Sciences
Biology and Life Sciences
biology.organism_classification
United States
Mycoses
Haplotypes
Genetic Loci
North America
Earth Sciences
People and places
Population Genetics
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....7f5c448fe02f38645b14c6824fe2f36d