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Qualitative and quantitative analyses of aconite alkaloids in Aconiti kusnezoffii Radix, and NO inhibitory activity evaluation of the alkaloid extracts
- Source :
- Food Science and Technology (2021), Food Science and Technology v.42 2022, Food Science and Technology (Campinas), Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA), instacron:SBCTA, Food Science and Technology, Issue: ahead, Published: 28 JUN 2021, Food Science and Technology, Volume: 42, Article number: e28521, Published: 28 JUN 2021
- Publication Year :
- 2022
- Publisher :
- FapUNIFESP (SciELO), 2022.
-
Abstract
- Aconiti kusnezoffii Radix (Caowu), a traditional Chinese medicine and Mongolian medicine, has been commonly used owing to its analgesic and anti-inflammatory effects. Here, we performed a qualitative analysis of aconite alkaloids (AAs) in raw and processed Aconiti kusnezoffii Radix by ultra-high-performance liquid chromatography (HPLC) electrospray ionization-tandem mass spectrometry. Furthermore, we quantified benzoylaconine, benzoylmesaconine, benzoylhypaconine, aconitine, mesaconitine, and hypaconitine in raw and processed Aconiti kusnezoffii Radix using a HPLC system coupled with a diode array detector. The analysis of AA fragmentation patterns in positive electrospray ionization mode revealed 155 AAs, including 6 with unambiguous identities, 109 with putative assignments, and 40 unknown compounds. Additionally, lipopolysaccharide-treated RAW264.7 macrophages were used to evaluate the anti-inflammatory properties of the alkaloid extracts of Aconiti kusnezoffii Radix. Importantly, alkaloid extracts of Aconiti kusnezoffii Radix at three different concentrations exhibited protective effect against lipopolysaccharide-induced inflammation. Thus, the proposed method can be used not only for the qualitative and quantitative analyses of Aconiti kusnezoffii Radix, but also for evaluating other Aconitum spp. Our study lays a foundation for the clinical application of poisonous Aconitum.
- Subjects :
- ultrahigh performance liquid chromatography-electrospray ionization tandem mass spectrometry
Traditional medicine
biology
Nutrition. Foods and food supply
Electrospray ionization
Alkaloid
aconite alkaloid
biology.organism_classification
High-performance liquid chromatography
qualitative and quantitative analyses
chemistry.chemical_compound
Qualitative analysis
chemistry
anti-inflammatory effect
Chromatography detector
T1-995
Aconitine
TX341-641
Radix
Aconiti kusnezoffii Radix
Technology (General)
Aconitum
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 1678457X and 01012061
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Food Science and Technology
- Accession number :
- edsair.doi.dedup.....7f5b4368c835d06ff6fb8ee0cac4c54c