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RHD positive among C/E+ and D-negative blood donors in Tunisia
- Source :
- Transfusion Clinique et Biologique. 21:320-323
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Purpose of the study The aim of this study was to investigate RHD alleles among Tunisian blood donors with D-negative phenotype and positive for C and/or E antigen. Patients and methods A total of 100 D-negative and C/E+ samples were analyzed by RHD genotyping using an initial test for RHD exon 10. In case of a positive reaction, further molecular investigations including real time quantitative PCR, allele specific PCR and nucleotide sequencing were done to elucidate the RHD involved mechanisms. Results Seventy-five percent of the studied samples lacked the RHD gene. Twenty-three percent carried the hybrid RHD-CE-D alleles (16 RHD-CE(3-7)-D , 5 RHD-CE(4-7)-D , 1 RHD-CE(4-8)-D , 1 RHD-CE(3-8)-D ) and 2% were weak D (1 weak D type 1 and 1 weak D type 5). Conclusion Our study proved the high frequency of RHD gene among serologically D-negative samples, positive for C and/or E antigen. Thus achieving systematically RHCE phenotyping in all transfusion centers on the Tunisian territory and considering blood donated from D-negative C/E+ persons as D-positive will be recommended to reduce anti-D allo-immunization.
- Subjects :
- Adult
Tunisia
Genotype
Clinical Biochemistry
RhD positive
Blood Donors
Biology
Rh Isoimmunization
Exon
Gene Frequency
Antigen
Isoantibodies
Humans
Allele
Genotyping
Gene
Alleles
Sequence Deletion
Genetics
Rh-Hr Blood-Group System
Biochemistry (medical)
Exons
Sequence Analysis, DNA
Hematology
Virology
Phenotype
Real-time polymerase chain reaction
Blood Grouping and Crossmatching
Variants of PCR
Gene Deletion
Subjects
Details
- ISSN :
- 12467820
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Transfusion Clinique et Biologique
- Accession number :
- edsair.doi.dedup.....7f572191e27eb2dfcbb7e600a61edae7