Development of charybdotoxin <scp>Q18F</scp> variant as a selective peptide blocker of neuronal <scp>BK</scp> (α + β4) channel for the treatment of epileptic seizures

Epilepsy is the results from the imbalance between inhibition and excitation in neural circuits, which is mainly treated by some chemical drugs with side effects. Gain-of-function of BK channels or knockout of its β4 subunit associates with spontaneous epilepsy. Currently, few reports were published about the efficacy of BK(α + β4) channel modulators in epilepsy prevention. Charybdotoxin is a non-specific inhibitor of BK and other K