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Differential ability of astroglia and neuronal cells to accumulate lead: dependence on cell type and on degree of differentiation

Authors :
Lora S. Lindahl
Laura Bird
Marie E. Legare
Evelyn Tiffany-Castiglioni
Ginger Mikeska
Gerald R. Bratton
Source :
Toxicological Sciences. 50:236-243
Publication Year :
1999
Publisher :
Oxford University Press (OUP), 1999.

Abstract

The apparent ability of astroglia to serve as a lead (Pb) sink in the mature brain may result from either their strategic location, between the blood-brain barrier and neurons, or from intrinsic differences between the ability of astroglia and neurons to accumulate this metal. This phenomenon may be dependent on the degree of cell differentiation. In order to address the latter possibility, Pb accumulation was compared among the following cell culture models: (1) mature and immature rat astroglia, (2) undifferentiated SY5Y human neuroblastoma cells and SY5Y cells differentiated with nerve growth factor, (3) immature rat astroglia grown in differently conditioned media, some of which induce partial differentiation, and (4) rat astroglia and SY5Y cells in co-culture. Astroglial cultures, prepared from 1-day-old rat cerebral hemispheres, were exposed to 1 microM Pb after either 14 (immature) or 21 (mature) days in culture. Pb content of the cells was measured by atomic absorption spectroscopy. Immature astroglia took up less Pb when glutathione (GSH) was added to the medium, suggesting that GSH may regulate Pb uptake in these cells. Undifferentiated neuroblastoma cells accumulated more Pb than did the differentiated ones. Astroglia accumulated up to 24 times more Pb than did neuronal cells. This ability was enhanced by exposure to conditioned medium from a neuroblastoma cell line, but not by endothelial cell-conditioned medium, although this medium induced the expression of a glutamate-activated Ca2+ response. Our findings are in agreement with in vivo studies, and thus validate the use of these cell-culture models for future studies on differential mechanisms of Pb uptake.

Details

ISSN :
10960929
Volume :
50
Database :
OpenAIRE
Journal :
Toxicological Sciences
Accession number :
edsair.doi.dedup.....7f3354c6b42fe0cd79784779888551b6
Full Text :
https://doi.org/10.1093/toxsci/50.2.236