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Cloning and nucleotide sequence of a cellulase gene, casA, from an alkalophilic Streptomyces strain
- Source :
- Gene. 65:229-238
- Publication Year :
- 1988
- Publisher :
- Elsevier BV, 1988.
-
Abstract
- A gene encoding an endo-type semi-alkaline cellulase was cloned from an alkalophilic Streptomyces strain in Streptomyces lividans, and its nucleotide sequence was determined. Downstream from the transcriptional start point, which was determined by high-resolution S1 mapping, an open reading frame of 388 amino acids (aa) was present. The N-terminal amino acid sequence of the mature enzyme determined by an Edman degradation procedure suggested that the cellulase had an extraordinarily long leader sequence of about 70 aa. Comparison with the leader sequences of endoglucosidase H from Streptomyces plicatus and the cellulase from Cellulomonas fimi suggested that the semi-alkaline cellulase was processed in two steps during maturation.
- Subjects :
- Transcription, Genetic
Molecular Sequence Data
DNA, Recombinant
Cellulase
Biology
Streptomyces
Genetics
Amino Acid Sequence
Cloning, Molecular
Promoter Regions, Genetic
Peptide sequence
Base Sequence
Edman degradation
Streptomycetaceae
Immunochemistry
Nucleic acid sequence
General Medicine
biology.organism_classification
Streptomyces plicatus
Open reading frame
Biochemistry
Genes, Bacterial
biology.protein
Plasmids
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....7f2ab353899c9b480c55eac7ff650c1e