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MicroRNA-15a promotes neuroblastoma migration by targeting reversion-inducing cysteine-rich protein with Kazal motifs (RECK) and regulating matrix metalloproteinase-9 expression
- Source :
- The FEBS journal. 280(3)
- Publication Year :
- 2012
-
Abstract
- In this study, we found that the expression of miR-15a was positively correlated with neuroblastoma (NB) clinical pathological stage and was negatively correlated with reversion-inducing cysteine-rich protein with Kazal motifs (RECK) expression. Using the enhanced green fluorescent protein (EGFP) reporter construct carrying the 3'-UTR of RECK, we identified RECK as a direct target of miR-15a. Suppression of miR-15a significantly decreased the migration ability of GI-LA-N and SK-N-SH cell lines, whereas overexpression of miR-15a increased the migration ability; these effects could be partly reversed by RECK inhibition or ectopic expression. Moreover, inhibition of miR-15a significantly increased secreted matrix metalloproteinase-9 expression in culture medium through regulating the expression of RECK. These findings provide new insights into the characteristics of the miR-15a-RECK-matrix metalloproteinase-9 axis in NB progression, especially in NB migration and invasion.
- Subjects :
- Blotting, Western
Green Fluorescent Proteins
GPI-Linked Proteins
Biochemistry
Kazal Motifs
Green fluorescent protein
Neuroblastoma
RNA interference
Cell Movement
Cell Line, Tumor
microRNA
Humans
Neoplasm Invasiveness
Neoplasm Metastasis
Molecular Biology
3' Untranslated Regions
Neoplasm Staging
Regulation of gene expression
Models, Genetic
Chemistry
Reverse Transcriptase Polymerase Chain Reaction
HEK 293 cells
Cell Biology
Molecular biology
Cell biology
Gene Expression Regulation, Neoplastic
MicroRNAs
HEK293 Cells
Spectrometry, Fluorescence
Matrix Metalloproteinase 9
Cell culture
Ectopic expression
RNA Interference
HeLa Cells
Subjects
Details
- ISSN :
- 17424658
- Volume :
- 280
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The FEBS journal
- Accession number :
- edsair.doi.dedup.....7f26ffe05880a62b2e17cdf4da50a3e6