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Optically-controlled bacterial metabolite for cancer therapy
- Source :
- Nature Communications, Nature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Bacteria preferentially accumulating in tumor microenvironments can be utilized as natural vehicles for tumor targeting. However, neither current chemical nor genetic approaches alone can fully satisfy the requirements on both stability and high efficiency. Here, we propose a strategy of “charging” bacteria with a nano-photocatalyst to strengthen their metabolic activities. Carbon nitride (C3N4) is combined with Escherichia coli (E. coli) carrying nitric oxide (NO) generation enzymes for photo-controlled bacterial metabolite therapy (PMT). Under light irradiation, photoelectrons produced by C3N4 can be transferred to E. coli to promote the enzymatic reduction of endogenous NO3– to cytotoxic NO with a 37-fold increase. In a mouse model, C3N4 loaded bacteria are perfectly accumulated throughout the tumor and the PMT treatment results in around 80% inhibition of tumor growth. Thus, synthetic materials-remodeled microorganism may be used to regulate focal microenvironments and increase therapeutic efficiency.<br />Targeting tumors with bacteria as vehicles for metabolite therapy suffers from low efficiency and robustness. Here, the authors combine carbon nitride with nitric oxide generation enzyme-positive E. coli for photo-controlled metabolite therapy (PMT) and observe increased effects both in vitro and in tumor-bearing mice.
- Subjects :
- Light
Science
Microorganism
Metabolite
General Physics and Astronomy
Apoptosis
02 engineering and technology
Nitric Oxide
010402 general chemistry
medicine.disease_cause
01 natural sciences
Article
General Biochemistry, Genetics and Molecular Biology
Nitric oxide
Mice
chemistry.chemical_compound
Cell Line, Tumor
Neoplasms
Nitriles
Escherichia coli
medicine
Animals
Humans
lcsh:Science
chemistry.chemical_classification
Mice, Inbred BALB C
Tumor microenvironment
Multidisciplinary
biology
General Chemistry
Photochemical Processes
021001 nanoscience & nanotechnology
biology.organism_classification
0104 chemical sciences
Biological Therapy
Oxidative Stress
Enzyme
chemistry
Biochemistry
Cell culture
lcsh:Q
Female
0210 nano-technology
Bacteria
Subjects
Details
- ISSN :
- 20411723
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....7f26e107e590f27c5533a014d4e6fa9d