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Unique features of the grapevine VvK5.1 channel support novel functions for outward K+ channels in plants
- Source :
- Journal of Experimental Botany, Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (21), pp.6181-6193. ⟨10.1093/jxb/erz341⟩, Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (21), ⟨10.1093/jxb/erz341⟩, Journal of Experimental Botany, Oxford University Press (OUP), 2019, ⟨10.1093/jxb/erz341⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; Grapevine (Vitis vinifera L.), one of the most important fruit crops, is a model plant for studying the physiology of fleshy fruits. Here, we report on the characterization of a new grapevine Shaker-type K+ channel, VvK5.1. Phylogenetic analysis revealed that VvK5.1 belongs to the SKOR-like subfamily. Our functional characterization of VvK5.1 in Xenopus oocytes confirms that it is an outwardly rectifying K+ channel that displays strict K+ selectivity. Gene expression level analyses by real-time quantitative PCR showed that VvK5.1 expression was detected in berries, roots, and flowers. In contrast to its Arabidopsis thaliana counterpart that is involved in K+ secretion in the root pericycle, allowing root to shoot K+ translocation, VvK5.1 expression territory is greatly enlarged. Using in situ hybridization we showed that VvK5.1 is expressed in the phloem and perivascular cells of berries and in flower pistil. In the root, in addition to being expressed in the root pericycle like AtSKOR, a strong expression of VvK5.1 is detected in small cells facing the xylem that are involved in lateral root formation. This fine and selective expression pattern of VvK5.1 at the early stage of lateral root primordia supports a role for outward channels to switch on cell division initiation.
- Subjects :
- 0106 biological sciences
root to shoot translocation
Potassium Channels
Cell division
Physiology
[SDV]Life Sciences [q-bio]
Plant Science
racine laterale
Plant Roots
01 natural sciences
phloem
F30 - Génétique et amélioration des plantes
root lateral primordium
Xenopus laevis
Gene Expression Regulation, Plant
Expression des gènes
Arabidopsis thaliana
Vitis
new tissue location sites
translocation transmembranaire
Lateral root formation
Phylogeny
Plant Proteins
0303 health sciences
root-to-shoot translocation
food and beverages
potassium Shaker channel
Physiologie végétale
Cell biology
localisation cellulaire
DNA, Complementary
new physiological properties
Flowers
Biology
03 medical and health sciences
grappe de raisin
Animals
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Primordium
RNA, Messenger
030304 developmental biology
shaker potassium channel
Lateral root
fungi
Xylem
vigne transgénique
biology.organism_classification
F60 - Physiologie et biochimie végétales
Pericycle
propriété physiologique
Vitis vinifera
Potassium
Oocytes
primordium
Phloème
Phloem
canaux potassiques shaker
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00220957 and 14602431
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany, Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (21), pp.6181-6193. ⟨10.1093/jxb/erz341⟩, Journal of Experimental Botany, Oxford University Press (OUP), 2019, 70 (21), ⟨10.1093/jxb/erz341⟩, Journal of Experimental Botany, Oxford University Press (OUP), 2019, ⟨10.1093/jxb/erz341⟩
- Accession number :
- edsair.doi.dedup.....7f17948c4351c2007e42f117721d237a