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Hierarchical biofabrication of biomimetic collagen-elastin vascular grafts with controllable properties via lyophilisation
- Source :
- Acta Biomaterialia. 112:52-61
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- This article describes the development of a hierarchical biofabrication technique suitable to create large but complex structures, such as vascular mimicking grafts, using facile lyophilisation technology amenable to multiple other biomaterial classes. The combination of three fabrication techniques together, namely solvent evaporation, lyophilisation, and crosslinking together allows highly tailorable structures from the microstructure up to the macrostructure, and with the ability to independently crosslink each layer it allows great flexibility to match desired native mechanical properties independently of the micro/macrostructure. We have demonstrated the flexibility of this biofabrication technique by independently optimising each of the layers to create a multi-layered arterial structure with tailored architectural and biophysical/biochemical properties using a collagen-elastin composite. Taken together, the facile biofabrication methodology developed has led to the development of a biomimetic bilayered scaffold suitable for use as a tissue engineered vascular graft (for haemodialysis access or peripheral/coronary bypass), or as an in vitro test platform to examine disease progression, pharmacological toxicity, or cardiovascular medical device testing. STATEMENT OF SIGNIFICANCE: The ability to grow large complex tissues such as blood vessels for transplantation is often hampered by the limitations of the selected biofabrication technique. Here, we sought to overcome some of the fabrication limitations for naturally occurring cardiovascular polymers (collagen/elastin) via a hierarchical approach to fabrication where each layer is built upon the previous. This approach enabled the flexibility to modify and tailor each layer's properties independently via control over polymer concentration, microstructure, and crosslinking. This simple approach facilitated us to fabricate multi-layered vascular grafts which were remodelled into high-density vascular tissue after 21-days. The fabrication approach could be translated to a myriad of other tissues while the engineered vascular graft could also be used as a test platform for drugs/medical devices or as a tissue engineering scaffold for vascular grafting for different indications.
- Subjects :
- Scaffold
Flexibility (anatomy)
Materials science
0206 medical engineering
Biomedical Engineering
02 engineering and technology
Biochemistry
Biomaterials
Tissue engineering
Biomimetics
medicine
Molecular Biology
Vascular tissue
Tissue Engineering
Tissue Scaffolds
biology
Biomaterial
General Medicine
021001 nanoscience & nanotechnology
020601 biomedical engineering
Blood Vessel Prosthesis
Elastin
Transplantation
medicine.anatomical_structure
biology.protein
Vascular Grafting
Collagen
0210 nano-technology
Biotechnology
Biofabrication
Biomedical engineering
Subjects
Details
- ISSN :
- 17427061
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Acta Biomaterialia
- Accession number :
- edsair.doi.dedup.....7efaf4c2171eb118562d8cfd8c3abc22