Back to Search
Start Over
The aliphatic amidase AmiE is involved in regulation of Pseudomonas aeruginosa virulence
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2017, 7, pp.41178. ⟨10.1038/srep41178⟩, Scientific Reports, 2017, 7, pp.41178. ⟨10.1038/srep41178⟩, Scientific reports, 7, Art.Nr. 41178
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- We have previously shown that the eukaryotic C-type natriuretic peptide hormone (CNP) regulates Pseudomonas aeruginosa virulence and biofilm formation after binding on the AmiC sensor, triggering the amiE transcription. Herein, the involvement of the aliphatic amidase AmiE in P. aeruginosa virulence regulation has been investigated. The proteome analysis of an AmiE over-producing strain (AmiE+) revealed an expression change for 138 proteins, including some that are involved in motility, synthesis of quorum sensing compounds and virulence regulation. We observed that the AmiE+ strain produced less biofilm compared to the wild type, and over-produced rhamnolipids. In the same line, AmiE is involved in P. aeruginosa motilities (swarming and twitching) and production of the quorum sensing molecules N-acyl homoserine lactones and Pseudomonas Quinolone Signal (PQS). We observed that AmiE overproduction reduced levels of HCN and pyocyanin causing a decreased virulence in different hosts (i.e. Dictyostelium discoideum and Caenorhabditis elegans). This phenotype was further confirmed in a mouse model of acute lung infection, in which AmiE overproduction resulted in an almost fully virulence decrease. Taken together, our data suggest that, in addition to its role in bacterial secondary metabolism, AmiE is involved in P. aeruginosa virulence regulation by modulating pilus synthesis and cell-to-cell communication.
- Subjects :
- Male
0301 basic medicine
Virulence regulation
MESH: Pseudomonas Infections/microbiology
Proteome
health care facilities, manpower, and services
MESH: Dictyostelium/microbiology
MESH: Virulence
medicine.disease_cause
MESH: Quorum Sensing
Pilus
chemistry.chemical_compound
MESH: Caenorhabditis elegans/microbiology
MESH: Amidohydrolases/metabolism
AmiE overproduction
Dictyostelium
MESH: Animals
Lung
health care economics and organizations
MESH: Pseudomonas aeruginosa/enzymology
Multidisciplinary
Virulence
Quorum Sensing
MESH: Proteome
Pseudomonas aeruginosa
Female
Life sciences
biology
MESH: Virulence Factors
Virulence Factors
030106 microbiology
Homoserine
MESH: Biofilms
Biology
Microbiology
Article
Amidohydrolases
Amidase
03 medical and health sciences
Pyocyanin
MESH: Mice, Inbred C57BL
ddc:570
Journal Article
medicine
Animals
Pseudomonas Infections
cardiovascular diseases
Caenorhabditis elegans
MESH: Lung/microbiology
MESH: Pseudomonas aeruginosa/pathogenicity
Biofilm
Bacteriology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Male
MESH: Pseudomonas Infections/enzymology
Mice, Inbred C57BL
Quorum sensing
chemistry
Biofilms
FOS: Biological sciences
Pseudomonas aeruginosa virulence
[SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
MESH: Female
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2017, 7, pp.41178. ⟨10.1038/srep41178⟩, Scientific Reports, 2017, 7, pp.41178. ⟨10.1038/srep41178⟩, Scientific reports, 7, Art.Nr. 41178
- Accession number :
- edsair.doi.dedup.....7ef6d60b3d9867f14773ecb97f5db3e6