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Extrahepatic Expression and Regulation of Protein C in the Mouse
- Source :
- The American Journal of Pathology. 153:547-555
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Activated protein C (APC) acts as an anticoagulant by inhibiting coagulation factors Va and VIIIa. Although the liver appears to be the primary site of protein C (PC) synthesis, the demonstration that other components of this system are produced extrahepatically raises the possibility that PC itself is synthesized in other tissues. We therefore used quantitative reverse transcription-polymerase chain reaction, in situ hybridization, and immunohistochemistry to screen various murine tissues for PC expression. Relatively high levels of PC mRNA were detected in the kidney (35% of liver) and testis (22% of liver). PC mRNA and antigen were demonstrated in tubular epithelial cells in the renal cortex, in spermatogenic cells in the testis, and in epithelial cells in the epididymis. Low but significant levels of PC mRNA were detected in the epididymis (1.7% of the level in liver), brain (1.1% of liver), and lung (0.8% of liver). PC antigen was demonstrated in bronchial epithelial cells in the lung, in pyramidal neurons in the cerebrum, and in Purkinje cells in the cerebellum. The extrahepatic expression of PC mRNA (ie, in the kidney) was significantly decreased in mice with renal disease (eg, in MRL lpr/lpr mice with autoimmune lupus nephritis, in db/db mice with diabetic nephropathy, and in endotoxin-treated mice with acute renal injury). The decreased renal expression of PC may contribute to the increased procoagulant potential of the kidney during septic and inflammatory processes and to the progression of kidney disease associated with these conditions.
- Subjects :
- Lipopolysaccharides
Male
medicine.medical_specialty
Renal cortex
Mice, Inbred Strains
In situ hybridization
Biology
Kidney
Polymerase Chain Reaction
Pathology and Forensic Medicine
Immunoenzyme Techniques
Mice
Antigen
Sepsis
Internal medicine
Testis
medicine
Animals
Diabetic Nephropathies
Tissue Distribution
RNA, Messenger
In Situ Hybridization
Nephritis
Kidney metabolism
medicine.disease
Molecular biology
Mice, Inbred C57BL
Endocrinology
medicine.anatomical_structure
Immunohistochemistry
Female
Kidney Diseases
Protein C
Regular Articles
medicine.drug
Kidney disease
Subjects
Details
- ISSN :
- 00029440
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- The American Journal of Pathology
- Accession number :
- edsair.doi.dedup.....7eeb6e710820e57a3a578b0a02f2c036