Characterization, differentiation and classification of aquatic humic matter separated with different sorbents: synchronous scanning fluorescence spectroscopy

Aquatic humic solutes were separated by the non-ionic macroporous XAD-8 and DAX-8 resins and a weakly basic DEAE cellulose anion exchanger from seven different fresh water sources. Synchronous fluorescence spectroscopy was applied for characterization, differentiation and classification of the different humic-solute aggregates. Fluorescence properties verified that humic-solute fractions isolated parallelly with the non-ionic XAD-8 and DAX-8 resins resembled very closely each other speaking strongly for their structural similarities. DAX-8 resin separated ca. 19% more aquatic humic matter than did the analogous XAD-8 resin. It was possible to tentatively differentiate the untreated water samples according to their fluorescent materials. Several distinct classes of chromophores were detected in both DOM and isolated humic fractions by the synchronous technique: λ ex / λ em 280/298, 330/348, 355/373, 400/418, 427/445, 460/478, 492/510 and 516/534 nm.