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Multiple Embryo Time-Lapse Imaging of Zebrafish Development
- Source :
- Methods in Molecular Biology ISBN: 9781603279765, Methods in Molecular Biology
- Publication Year :
- 2009
- Publisher :
- Humana Press, 2009.
-
Abstract
- Understanding the dynamics of developmental and cellular processes requires documentation of their changes with appropriate temporal and spatial resolution. Furthermore, simultaneous recording from a population of embryos under identical conditions allows statistical estimates of precision and variability to be made. This chapter describes a protocol for time-lapse microscopy of multiple embryos in parallel developing under tightly controlled conditions. This method is currently best suited to follow tissue-scale morphogenetic movements with temporal resolution in the minute range, for hours or even days. Applications of the method include the comparison of the dynamics of a process of interest between groups of wild-type embryos and their mutant siblings or between embryos treated with different chemical compounds. Temperature control allows for the investigation of the temperature dependence of a process of interest.
- Subjects :
- Time Factors
Population
embryo
Growth
animal embryo
Article
Time
histology
03 medical and health sciences
Computer-Assisted
0302 clinical medicine
Image processing
Developmental biology
zebra fish
Animals
animal
Time-Lapse Imaging
education
Zebrafish
030304 developmental biology
0303 health sciences
education.field_of_study
Nonmammalian
biology
development and aging
Methodology
temperature
embryo development
Embryo
Anatomy
zebrafish
biology.organism_classification
Temporal resolution
Embryonic development
Biological system
030217 neurology & neurosurgery
Subjects
Details
- ISBN :
- 978-1-60327-976-5
- ISBNs :
- 9781603279765
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781603279765, Methods in Molecular Biology
- Accession number :
- edsair.doi.dedup.....7ecf5d77842f4a075893e4e4e23e2e43