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Sequence variation and mRNA expression of the TWIST1 gene in cats with mammary hyperplasia and neoplasia
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- In humans, a germline mutation (c.309C>G) in the TWIST1 oncogene may predispose to breast cancer and its expression has been associated with tumour progression and metastasis. In this study, the feline TWIST1 gene was screened for sequence variations in 37 neoplastic and eight hyperplastic mammary gland lesions from cats. In addition, mRNA levels were examined in 15 mammary tumours and three cases of mammary hyperplasia by quantitative real-time reverse-transcriptase PCR. Feline mammary carcinomas had significantly lower levels of expression of TWIST1 mRNA than benign mammary tumours. No variations were identified in the TWIST1 coding region in feline mammary tumours and the mutation described in humans was not detected. However, two germline variants in the TWIST1 gene intron were identified in four and three carcinomas, respectively: GQ167299:g.535delG and GQ167299:g.460C>T. There was no association between these sequence alterations and TWIST1 mRNA levels.
- Subjects :
- Pathology
medicine.medical_specialty
animal structures
040301 veterinary sciences
Mammary gland
Mammary Neoplasms, Animal
Biology
Cat Diseases
Germline
Metastasis
0403 veterinary science
03 medical and health sciences
0302 clinical medicine
Germline mutation
medicine
Animals
Coding region
RNA, Messenger
DNA Primers
Regulation of gene expression
General Veterinary
Oncogene
Reverse Transcriptase Polymerase Chain Reaction
Twist-Related Protein 1
04 agricultural and veterinary sciences
Hyperplasia
medicine.disease
3. Good health
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cats
Cancer research
Female
Animal Science and Zoology
Subjects
Details
- ISSN :
- 10900233
- Volume :
- 191
- Database :
- OpenAIRE
- Journal :
- The Veterinary Journal
- Accession number :
- edsair.doi.dedup.....7ecd327c76b1c9f66b74c4ff16449203