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Induction of cdc2a and cyc1At expression in Arabidopsis thaliana during early phases of nematode-induced feeding cell formation
- Source :
- Plant Journal, Plant Journal, Wiley, 1996, 10 (6), pp.1037-1043, Scopus-Elsevier
- Publication Year :
- 1996
-
Abstract
- Summary Root-knot and cyst nematodes are plant parasites that induce large multinucleated feeding cells in the roots of their hosts. Cytological observations have shown that root-knot nematodes induce giant cells by cycles of mitosis without cytokinesis whereas cyst nematodes provoke cell wall degradation leading to the formation of a large syncytium. This study was intended to characterize and compare the ability of both types of nematodes to induce progression through the cell cycle. For this purpose, the expression, upon nematode infection, of two cell cycle markers was followed: a marker for division competence, the cyclin-dependent kinase cdc2a and a marker for the G2 phase, the mitotic cyclin cyc1At. For both types of nematodes, transcriptional activation of these markers was correlated with early phases of feeding cell development. Using molecular markers, it was thus possible to confirm and extend the observations of repeated mitosis in root-knot nematode-induced giant cells. Surprisingly, promoter activation of both cdc2a and cyc1At markers was also found upon cyst nematode infection, in feeding cells in which mitosis has not been clearly reported. Incorporation of tritiated thymidine in these syncytia confirms that they progress through the S phase of the cell cycle. One possibility is that cyst nematodes induce cycles of DNA endoreduplication shunting the M phase. Despite obvious differences in ontogeny, common molecular mechanisms, involving cycles of DNA endoreduplication and cdc2a and cyc1At expression, might thus be involved in the formation of a giant cell or a syncytium.
- Subjects :
- 0106 biological sciences
Arabidopsis
Plant Science
Biology
01 natural sciences
Plant Roots
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Secernentea Infections
Gene Expression Regulation, Plant
Genes, Reporter
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
Cyclins
CDC2 Protein Kinase
Genetics
Animals
Nematode Infections
Mitosis
ComputingMilieux_MISCELLANEOUS
S phase
030304 developmental biology
Plant Diseases
0303 health sciences
Syncytium
Cell growth
Cell Cycle
Cell Biology
Cell cycle
Plants, Genetically Modified
Antigens, Differentiation
Cell biology
DNA endoreduplication
Giant cell
Cytokinesis
010606 plant biology & botany
Subjects
Details
- ISSN :
- 09607412 and 1365313X
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The Plant journal : for cell and molecular biology
- Accession number :
- edsair.doi.dedup.....7ec6c32c78a1f2743af4bfc5102cb862