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Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients
- Source :
- Cellular Physiology and Biochemistry, Vol 49, Iss 5, Pp 1804-1812 (2018)
- Publication Year :
- 2018
- Publisher :
- S. Karger AG, 2018.
-
Abstract
- Background/Aims: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. Methods: Analysis of the oral microbiota from 12 gout patients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR). Results: Our analysis indicated that both gout and HUA significantly altered the composition of the oral microbiome in patients. Patients with gout or HUA had significantly greater levels of salivary Prevotella intermedia but significantly lower levels of Serratia marcescens than healthy control subjects. Conclusion: We demonstrated the association between the oral microbiome and gout and HUA for the first time. In particular, 16S sequencing and qPCR analysis revealed significantly higher levels of oral Prevotella intermedia in gout/HUA patients, which suggests that these patients might be at risk for the development of periodontitis.
- Subjects :
- 16S rDNA sequencing
Adult
Male
musculoskeletal diseases
0301 basic medicine
congenital, hereditary, and neonatal diseases and abnormalities
medicine.medical_specialty
Gout
Physiology
Hyperuricemia
Prevotella intermedia
Gastroenterology
lcsh:Physiology
Deep sequencing
lcsh:Biochemistry
03 medical and health sciences
RNA, Ribosomal, 16S
Internal medicine
medicine
Humans
lcsh:QD415-436
Microbiome
Serratia marcescens
Aged
Periodontitis
Mouth
lcsh:QP1-981
biology
business.industry
Microbiota
nutritional and metabolic diseases
Middle Aged
medicine.disease
biology.organism_classification
stomatognathic diseases
030104 developmental biology
Oral microbiota
Streptococcus anginosus
Case-Control Studies
Female
Oral Microbiome
business
Subjects
Details
- ISSN :
- 14219778 and 10158987
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Cellular Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....7ebae616f8e17075ae1eb04087294ef5