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Tubuloreticular Inclusions and Paired Cisternae Induced in Human Lymphocytes Cultured withStaphylococcus AureusCowan 1
- Source :
- Ultrastructural Pathology. 8:155-163
- Publication Year :
- 1985
- Publisher :
- Informa UK Limited, 1985.
-
Abstract
- Tubuloreticular inclusions (TRI) and paired cisternae (PC) were induced in lymphocytes of normal individuals after incubation with Staphylococcus aureus Cowan 1. TRI were initially detected in lymphoid cells on day 2 (48-h culture). The frequency of TRI-positive cell sections on day 5 increased about twofold over those on days 2-4. On day 7, TRI were predominantly seen in lymphoplasmacytoid cells or plasmacytoid cells, with an incidence of up to 18% of sections. The regions in these cells were most extensive and anastomosed with the cisternae of adjacent well-developed rough endoplasmic reticulum (RER). TRI formation appears not to be essential for mitogen-induced B-cell differentiation to plasmacytoid cells, because pokeweed mitogen (PWM) failed to induce TRI. The diverse expressions of TRI induction between these two mitogens may be due to a difference in B-cell activation mechanisms. Paired cisternae were observed in a great majority of mitotic cells at various stages. These were encountered most frequently on day 4. PC were also seen in the PWM-stimulated culture. Our observations suggest that PC formation may be related to new formation of RER as well as to reconstruction of the nuclear envelope.
- Subjects :
- Staphylococcus aureus
Endoplasmic reticulum
Cell
Biology
medicine.disease_cause
Virology
Molecular biology
Pathology and Forensic Medicine
Organoids
Microscopy, Electron
medicine.anatomical_structure
Structural Biology
Interferon
medicine
Humans
Lymphocytes
Mitosis
Incubation
Cells, Cultured
medicine.drug
Subjects
Details
- ISSN :
- 15210758 and 01913123
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Ultrastructural Pathology
- Accession number :
- edsair.doi.dedup.....7eb1ae1fc41e147773101079508b0c10