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A novel angiotensin I-converting enzyme mutation (S333W) impairs N-domain enzymatic cleavage of the anti-fibrotic peptide, AcSDKP
- Source :
- PLoS One, PLoS ONE, PLoS ONE, Vol 9, Iss 2, p e88001 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- Background Angiotensin I-converting enzyme (ACE) has two functional N- and C-domain active centers that display differences in the metabolism of biologically-active peptides including the hemoregulatory tetrapeptide, Ac-SDKP, hydrolysed preferentially by the N domain active center. Elevated Ac-SDKP concentrations are associated with reduced tissue fibrosis. Results We identified a patient of African descent exhibiting unusual blood ACE kinetics with reduced relative hydrolysis of two synthetic ACE substrates (ZPHL/HHL ratio) suggestive of the ACE N domain center inactivation. Inhibition of blood ACE activity by anti-catalytic mAbs and ACE inhibitors and conformational fingerprint of blood ACE suggested overall conformational changes in the ACE molecule and sequencing identified Ser333Trp substitution in the N domain of ACE. In silico analysis demonstrated S333W localized in the S1 pocket of the active site of the N domain with the bulky Trp adversely affecting binding of ACE substrates due to steric hindrance. Expression of mutant ACE (S333W) in CHO cells confirmed altered kinetic properties of mutant ACE and conformational changes in the N domain. Further, the S333W mutant displayed decreased ability (5-fold) to cleave the physiological substrate AcSDKP compared to wild-type ACE. Conclusions and Significance A novel Ser333Trp ACE mutation results in dramatic changes in ACE kinetic properties and lowered clearance of Ac-SDKP. Individuals with this mutation (likely with significantly increased levels of the hemoregulatory tetrapeptide in blood and tissues), may confer protection against fibrosis.
- Subjects :
- ACE inhibitors
Substitution mutation
Mutant
lcsh:Medicine
Peptide
Cardiovascular
Biochemistry
Membrane proteins
lcsh:Science
Peptide sequence
chemistry.chemical_classification
Multidisciplinary
biology
Immunochemistry
Hydrolysis
CHO cells
Enzymes
Mutation detection
Blood
Medicine
Oligopeptides
Research Article
Molecular Sequence Data
Peptidyl-Dipeptidase A
Cell Line
Cricetulus
Genetic Mutation
Renin–angiotensin system
Genetics
Animals
Humans
Amino Acid Sequence
Biology
Enzyme Kinetics
Clinical Genetics
Tetrapeptide
Point mutation
Crystal structure
lcsh:R
Personalized Medicine
Active site
Proteins
Molecular biology
Fibrosis
Kinetics
Enzyme
Metabolism
chemistry
Mutation
Enzyme Structure
biology.protein
lcsh:Q
Peptides
Sequence Alignment
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS One, PLoS ONE, PLoS ONE, Vol 9, Iss 2, p e88001 (2014)
- Accession number :
- edsair.doi.dedup.....7e9e6969c8f799e8faf237052806eb1b
- Full Text :
- https://doi.org/10.1371/journal.pone.0088001