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Metabolizable Near-Infrared-II Nanoprobes for Dynamic Imaging of Deep-Seated Tumor-Associated Macrophages in Pancreatic Cancer
- Source :
- ACS nano. 15(6)
- Publication Year :
- 2021
-
Abstract
- Tumor-associated macrophages (TAMs) play a crucial part in cancer evolution. Dynamic imaging of TAMs is of great significance for treatment outcome evaluation and precision tumor therapy. Currently, most fluorescence nanoprobes tend to accumulate in the liver and are difficult to metabolize, which leads to strong background signals and inadequate imaging quality of TAMs nearby the liver such as pancreatic cancer. Herein, we aim to develop metabolizable dextran-indocyanine green (DN-ICG) nanoprobes in the second near-infrared window (NIR-II, 1 000-1 700 nm) for dynamic imaging of TAMs in pancreatic cancer. Compared to free ICG, the NIR-II fluorescence intensity of DN-ICG nanoprobes increased by 279% with significantly improved stability. We demonstrated that DN-ICG nanoprobes could specifically target TAMs through the interaction of dextran with specific ICAM-3-grabbing nonintegrin related 1 (SIGN-R1), which were highly expressed in TAMs. Subsequently, DN-ICG nanoprobes gradually metabolized in the liver yet remained in pancreatic tumor stroma in mouse models, achieving a high signal-to-background ratio (SBR = 7) in deep tissue (∼0.5 cm) NIR-II imaging of TAMs. Moreover, DN-ICG nanoprobes could detect dynamic changes of TAMs induced by low-dose radiotherapy and zoledronic acid. Therefore, the highly biocompatible and biodegradable DN-ICG nanoprobes harbor great potential for precision therapy in pancreatic cancer.
- Subjects :
- Indocyanine Green
medicine.medical_treatment
Dynamic imaging
General Physics and Astronomy
02 engineering and technology
010402 general chemistry
01 natural sciences
chemistry.chemical_compound
Mice
stomatognathic system
Stroma
Pancreatic tumor
Pancreatic cancer
Tumor-Associated Macrophages
medicine
Animals
General Materials Science
skin and connective tissue diseases
Spectroscopy, Near-Infrared
Chemistry
Optical Imaging
General Engineering
021001 nanoscience & nanotechnology
medicine.disease
0104 chemical sciences
Radiation therapy
Pancreatic Neoplasms
Fluorescence intensity
Cancer evolution
Cancer research
0210 nano-technology
Indocyanine green
Subjects
Details
- ISSN :
- 1936086X
- Volume :
- 15
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- ACS nano
- Accession number :
- edsair.doi.dedup.....7e9b8213b46f672fed1eb3566b9bc0ea