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Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification
- Source :
- PLoS ONE, Vol 16, Iss 5, p e0251119 (2021), PLoS ONE
- Publication Year :
- 2021
-
Abstract
- Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the main pathogenic microorganisms causing sexually transmitted infections. In this study, a multiplex thermostable recombinase polymerase amplification-lateral flow detection (RPA-LFD) assay was established, and the reaction conditions such as the ratio of primer concentration, magnesium ion concentration, amplification time and template DNA concentration in the multiplex RPA reaction were optimized. The optimized multiplex RPA-LFD method was used to detect both CT and NG positive control plasmids, and it was found that the LFD could be used to obtain visible results when the plasmid copy number was only 200. The sensitivity of the multiplex RPA-LFD method used for clinical samples was 85.62 (95% CI at 53.66–97.29) for NG detection and 90.90 (95% CI at 57.12–99.52) for CT detection.
- Subjects :
- Eye Diseases
Eye Infections
Reaction Systems
Recombinase Polymerase Amplification
Artificial Gene Amplification and Extension
Neonatal Conjunctivitis
Chlamydia trachomatis
medicine.disease_cause
Polymerase Chain Reaction
Systems Science
Chlamydia Infection
law.invention
Gonorrhea
Medical Conditions
law
Pregnancy
Medicine and Health Sciences
Magnesium
Multiplex
Signal Amplification
Magnesium ion
Polymerase chain reaction
Polymerase
Multidisciplinary
food and beverages
Chemistry
Infectious Diseases
Physical Sciences
Engineering and Technology
Medicine
Female
Research Article
Chemical Elements
DNA, Bacterial
Computer and Information Sciences
Science
Sexually Transmitted Diseases
Biology
Research and Analysis Methods
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Recombinases
medicine
Humans
Molecular Biology Techniques
Molecular Biology
Biology and Life Sciences
Chlamydia Infections
Conjunctivitis
Virology
Neisseria gonorrhoeae
Ophthalmology
Signal Processing
biology.protein
Pregnant Women
Primer (molecular biology)
Mathematics
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....7e8d5623941fee3b6cba9bdcbcdaa6af