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Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior
- Source :
- Beilstein Journal of Nanotechnology, Vol 10, Iss 1, Pp 2329-2337 (2019), Beilstein Journal of Nanotechnology
- Publication Year :
- 2019
- Publisher :
- Beilstein-Institut, 2019.
-
Abstract
- The stiffness and the topography of the substrate at the cell–substrate interface are two key properties influencing cell behavior. In this paper, atomic force acoustic microscopy (AFAM) is used to investigate the influence of substrate stiffness and substrate topography on the responses of L929 fibroblasts. This combined nondestructive technique is able to characterize materials at high lateral resolution. To produce substrates of tunable stiffness and topography, we imprint nanostripe patterns on undeveloped and developed SU-8 photoresist films using electron-beam lithography (EBL). Elastic deformations of the substrate surfaces and the cells are revealed by AFAM. Our results show that AFAM is capable of imaging surface elastic deformations. By immunofluorescence experiments, we find that the L929 cells significantly elongate on the patterned stiffness substrate, whereas the elasticity of the pattern has only little effect on the spreading of the L929 cells. The influence of the topography pattern on the cell alignment and morphology is even more pronounced leading to an arrangement of the cells along the nanostripe pattern. Our method is useful for the quantitative characterization of cell–substrate interactions and provides guidance for the tissue regeneration therapy in biomedicine.
- Subjects :
- nanopattern
Materials science
General Physics and Astronomy
Atomic force acoustic microscopy
02 engineering and technology
Lateral resolution
Photoresist
010402 general chemistry
lcsh:Chemical technology
01 natural sciences
lcsh:Technology
Full Research Paper
stiffness
topography
medicine
Substrate stiffness
Nanotechnology
cell growth
General Materials Science
lcsh:TP1-1185
Electrical and Electronic Engineering
Elasticity (economics)
Composite material
SU-8 photoresist
lcsh:Science
Lithography
lcsh:T
Stiffness
su-8 photoresist
021001 nanoscience & nanotechnology
lcsh:QC1-999
0104 chemical sciences
atomic force acoustic microscopy (afam)
Nanoscience
lcsh:Q
medicine.symptom
0210 nano-technology
lcsh:Physics
Subjects
Details
- Language :
- English
- ISSN :
- 21904286
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Beilstein Journal of Nanotechnology
- Accession number :
- edsair.doi.dedup.....7e8925c3327e385cf9989c4e933f58b1