Back to Search
Start Over
High-Performance Thin-Layer Chromatography for Rutin, Chlorogenic Acid, Caffeic Acid, Ursolic Acid, and Stigmasterol Analysis in Periploca aphylla Extracts
- Source :
- Separations, Vol 8, Iss 44, p 44 (2021), Separations, Volume 8, Issue 4
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Periploca aphylla (PA), an interesting Saudi medicinal plant, is used in folk medicine to treat urticaria, cerebral fever, tumors, and swelling. To prove its use in folk medicine, two different extracts from the aerial parts of the plant: chloroform P-1, and n-butanol P-2 were subjected to biological assays to screen peroxisome proliferator-activated receptors (PPARα and PPARγ) agnostic, anti-inflammatory, antioxidant, cytotoxic, and estrogenic activities. In addition, five bioactive secondary metabolites were isolated from the aerial parts of the plant: rutin, chlorogenic acid, caffeic acid, ursolic acid, and stigmasterol. P-1 and P-2 decreased cellular oxidative stress by 47.0% and 62.0%, respectively, compared to the standard drug quercetin, while one of the compounds rutin PA-1 isolated from P-1 extract and significantly decreased cellular oxidative stress by 67.0% compared to quercetin (75.0%). P-1 and P-2 also significantly activated PPARγ agnostic. P-1 and P-2 did not inhibit nuclear factor kappa B and inducible nitric oxide synthase activity and showed no cytotoxic or estergenic effects on four human cancer cell lines. In this study, both extracts were standardized using high-performance thin-layer chromatography (HPTLC). RP-HPTLC showed sharp and compact bands of rutin (Rf = 0.09), caffeic acid (Rf = 0.25), and chlorogenic acid (Rf = 0.39) scanned at λmax = 340 nm using the water: methanol (60:40 v/v) mobile phase. At λmax = 539 nm ursolic acid (Rf = 0.20) and stigmasterol (Rf = 0.48) were scanned using the chloroform: methanol (98:2 v/v) as NP-HPTLC mobile phase. Therefore, the developed RP- and NP-HPTLC systems are a precise, sensitive, and specific analytical tool for the quantification of compounds isolated from PA, which can be used as phytomarkers for taxonomical identification and assessment of PA.
- Subjects :
- Antioxidant
antioxidant
PPARγ
medicine.medical_treatment
Filtration and Separation
01 natural sciences
Reverse phase (RP-)
Analytical Chemistry
HPTLC
lcsh:Chemistry
chemistry.chemical_compound
Rutin
Chlorogenic acid
Ursolic acid
medicine
Caffeic acid
Normal Phase (NP-)
High performance thin layer chromatography
Periploca aphylla
Stigmasterol
Chromatography
010405 organic chemistry
010401 analytical chemistry
lcsh:QC1-999
0104 chemical sciences
chemistry
lcsh:QD1-999
compounds
Quercetin
lcsh:Physics
Subjects
Details
- Language :
- English
- ISSN :
- 22978739
- Volume :
- 8
- Issue :
- 44
- Database :
- OpenAIRE
- Journal :
- Separations
- Accession number :
- edsair.doi.dedup.....7e822ca1460107eb33cb1499439012ec