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Effect of mutant p27kip1 gene on human cholangiocarcinoma cell line, QBC939
- Publication Year :
- 2008
- Publisher :
- The WJG Press and Baishideng, 2008.
-
Abstract
- AIM: To investigate the effects of exogenously mutated p27kip1 (p27) on proliferation and apoptosis of human cholangiocarcinoma cell line, QBC939 in vivo. METHODS: Adenoviral vectors were used to transfect mutated p27 cDNA into human QBC939 cell line. Expression of p27 was detected by RT-PCR. Western blot. Cell growth, morphological change, cell cycle, apoptosis and cloning formation were determined by MTT assay and flow cytometry. RESULTS: The expression of p27 protein and mRNA was increased significantly in QBC939 cell line transfected with Ad-p27mt. The transfer of Ad-p27mt could significantly inhibit the growth of QBC939 cells, decrease the cloning formation rate and induce apoptosis. p27 over expression caused cell cycle arrest at G0/G1 phase 72 h after infection with Ad-p27mt. CONCLUSION: p27 may cause cell cycle arrest at G0/G1 phase and subsequently lead to apoptosis. Recombinant adenovirus expressing mutant p27 may be potentially useful in gene therapy for cholangiocarcinoma.
- Subjects :
- Cell cycle checkpoint
Apoptosis
Biology
Transfection
digestive system
Flow cytometry
Adenoviridae
Cholangiocarcinoma
Cell Line, Tumor
medicine
Humans
MTT assay
RNA, Messenger
RNA, Neoplasm
neoplasms
Cell Proliferation
medicine.diagnostic_test
Base Sequence
Cell growth
Cell Cycle
Gastroenterology
Intracellular Signaling Peptides and Proteins
General Medicine
Cell cycle
Molecular biology
digestive system diseases
Bile Duct Neoplasms
Cell culture
Mutation
Rapid Communication
Cyclin-Dependent Kinase Inhibitor p27
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7e798b46103d8b45bfdd562ed6b97457