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Effect of mutant p27kip1 gene on human cholangiocarcinoma cell line, QBC939

Authors :
Wei-Yu Wang
Yong-Jun Chen
Jian Luo
Sheng-Quan Zou
Publication Year :
2008
Publisher :
The WJG Press and Baishideng, 2008.

Abstract

AIM: To investigate the effects of exogenously mutated p27kip1 (p27) on proliferation and apoptosis of human cholangiocarcinoma cell line, QBC939 in vivo. METHODS: Adenoviral vectors were used to transfect mutated p27 cDNA into human QBC939 cell line. Expression of p27 was detected by RT-PCR. Western blot. Cell growth, morphological change, cell cycle, apoptosis and cloning formation were determined by MTT assay and flow cytometry. RESULTS: The expression of p27 protein and mRNA was increased significantly in QBC939 cell line transfected with Ad-p27mt. The transfer of Ad-p27mt could significantly inhibit the growth of QBC939 cells, decrease the cloning formation rate and induce apoptosis. p27 over expression caused cell cycle arrest at G0/G1 phase 72 h after infection with Ad-p27mt. CONCLUSION: p27 may cause cell cycle arrest at G0/G1 phase and subsequently lead to apoptosis. Recombinant adenovirus expressing mutant p27 may be potentially useful in gene therapy for cholangiocarcinoma.

Details

Language :
English
Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....7e798b46103d8b45bfdd562ed6b97457