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Biosynthesis of bioactive tamarixetin in recombinant Escherichia coli
- Source :
- Biotechnology and Applied Biochemistry. 68:531-537
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Tamarixetin, a monomethylated derivative of quercetin, has been reported to possess many important biological activities. In the present study, a whole cell biotransformation system was used for regiospecific methylation of quercetin to produce 4'-O-methylated quercetin (tamarixetin) using methyltransferase from Streptomyces sp. KCTC 0041BP in Escherichia coli Bl21 (DE3). Its production was enhanced by adding a plasmid containing S-adenosine-l-methionine (SAM) synthase from E. coli K12 (MetK) with subsequent feeding of l-methionine and glycerol in the culture. The best condition produced ∼279 μM (88.2 mg/L) of tamarixetin. The biological activity of tamarixetin was tested and compared with quercetin, 7-O-methylated quercetin, and 3-O-methylated quercetin. Results showed that the growth of all tested cancer cell lines (AGS, B16F10, C6, and HeLa) were inhibited by tamarixetin more effectively than other methylated derivatives of quercetin or quercetin. Tamarixetin also exhibited the best antimelanogenic activity among all compounds tested.
- Subjects :
- 0106 biological sciences
Biomedical Engineering
Antineoplastic Agents
Bioengineering
Disaccharides
medicine.disease_cause
01 natural sciences
Applied Microbiology and Biotechnology
Streptomyces
HeLa
Mice
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Biotransformation
010608 biotechnology
Drug Discovery
Escherichia coli
Tumor Cells, Cultured
medicine
Animals
Humans
heterocyclic compounds
Cell Proliferation
030304 developmental biology
0303 health sciences
Dose-Response Relationship, Drug
Molecular Structure
biology
Process Chemistry and Technology
Biological activity
Methyltransferases
General Medicine
biology.organism_classification
Tamarixetin
chemistry
Biochemistry
Molecular Medicine
Quercetin
Drug Screening Assays, Antitumor
Biotechnology
Subjects
Details
- ISSN :
- 14708744 and 08854513
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Applied Biochemistry
- Accession number :
- edsair.doi.dedup.....7e56651282baa8b0e647617964f22cfa
- Full Text :
- https://doi.org/10.1002/bab.1958