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Biosynthesis of bioactive tamarixetin in recombinant Escherichia coli

Authors :
Jae Kyung Sohng
Dipesh Dhakal
Hye Jin Jung
Narae Jung
Sanghun Lee
Sumangala Darsandhari
Biplav Shrestha
Source :
Biotechnology and Applied Biochemistry. 68:531-537
Publication Year :
2020
Publisher :
Wiley, 2020.

Abstract

Tamarixetin, a monomethylated derivative of quercetin, has been reported to possess many important biological activities. In the present study, a whole cell biotransformation system was used for regiospecific methylation of quercetin to produce 4'-O-methylated quercetin (tamarixetin) using methyltransferase from Streptomyces sp. KCTC 0041BP in Escherichia coli Bl21 (DE3). Its production was enhanced by adding a plasmid containing S-adenosine-l-methionine (SAM) synthase from E. coli K12 (MetK) with subsequent feeding of l-methionine and glycerol in the culture. The best condition produced ∼279 μM (88.2 mg/L) of tamarixetin. The biological activity of tamarixetin was tested and compared with quercetin, 7-O-methylated quercetin, and 3-O-methylated quercetin. Results showed that the growth of all tested cancer cell lines (AGS, B16F10, C6, and HeLa) were inhibited by tamarixetin more effectively than other methylated derivatives of quercetin or quercetin. Tamarixetin also exhibited the best antimelanogenic activity among all compounds tested.

Details

ISSN :
14708744 and 08854513
Volume :
68
Database :
OpenAIRE
Journal :
Biotechnology and Applied Biochemistry
Accession number :
edsair.doi.dedup.....7e56651282baa8b0e647617964f22cfa
Full Text :
https://doi.org/10.1002/bab.1958