MOESM3 of Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR

Additional file 3: Figure S3. Amplification plot of dilution experiment by BNA-clamp PCR method. Wild-type control DNA was spiked in the Tru-Q 7 (1.3% Tier) Reference Standard. BNA-clamp PCR was performed using serial dilution DNA. (A) DNA containing KRAS mutation at codon 12/13 (dilution range: 1–33% variant allele fraction) and (B) BRAF mutation at codon 600 (dilution range: 0.4–12% variant allele fraction).