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Genomic response programs of Candida albicans following protoplasting and regeneration
- Source :
- Recercat. Dipósit de la Recerca de Catalunya, instname
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Transcription profiling of Candida albicans cells responding to the elimination of the wall (protoplasts) and posterior regeneration was explored. DNA microarrays were used to measure changes in the expression of 6039 genes, and the upregulated genes during regeneration at 28 degrees C were assigned to fourteen categories. A total of 407 genes were upregulated during the process, of which 144 reached a maximum after 1 h. MKC1, a gene encoding a member of the regulatory pathway involved in cell wall integrity was overexpressed. Time-dependent expression divided the genes into 40 clusters. Clusters 1-19 were highly expressed initially (time 0) and downregulated following incubation, whereas transcription of the genes grouped into clusters 20-40 showed the opposite behaviour. These results suggest that the first clusters group genes permitting the cell adaptation to a sub-optimal environment due to removal of the wall, whereas the second group represents genes required for protoplasts regeneration after shifted to optimal conditions from 4 to 28 degrees C.
- Subjects :
- Regulation of gene expression
biology
Gene Expression Profiling
Protoplasts
biology.organism_classification
Microbiology
Genome
Molecular biology
Fungal Proteins
Gene expression profiling
Cell Wall
Transcription (biology)
Gene Expression Regulation, Fungal
Candida albicans
Gene expression
Genetics
Cluster Analysis
Regeneration
Genome, Fungal
DNA microarray
Gene
Oligonucleotide Array Sequence Analysis
Subjects
Details
- ISSN :
- 10871845
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Fungal Genetics and Biology
- Accession number :
- edsair.doi.dedup.....7e4349ed9c0b3ff51b8af1b24fbcc6f4