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Role of Kupffer cells in gut ischemia/reperfusion-induced hepatic microvascular dysfunction in mice
- Source :
- Hepatology. 26:1499-1505
- Publication Year :
- 1997
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1997.
-
Abstract
- Kupffer cells (KCs) have been implicated in the leukocyte recruitment and microvascular dysfunction associated with liver inflammation. The overall objective of this study was to assess the role of KCs in the leukocyte adhesion and oxidative stress elicited in the liver by gut ischemia/reperfusion (I/R). The accumulation of rhodamine-6G-labeled leukocytes and the number of nonperfused sinusoids (NPS) were monitored (by intravital microscopy) in mouse liver for 1 hour after a 15-minute period of normothermic intestinal ischemia. Autofluorescence of pyridine nucleotide [NAD(P)H] was measured as an index of mitochondrial O2 consumption and redox status. Leukostasis, as well as increases in NPS and NAD(P)H autofluorescence (indicating hypoxia), were observed in the liver at 60 minutes after gut I/R. Pretreatment with gadolinium chloride (GdCl3), which reduces KC function, attenuated the liver leukostasis and NPS elicited by gut I/R. The platelet activating factor (PAF) antagonist, WEB2086, and a tumor necrosis factor (TNF)-alpha-specific antibody were also effective in attenuating the gut I/R-induced leukostasis and NAD(P)H autofluorescence. The findings of this study suggest that KCs play an important role in mediating the leukocyte recruitment, impaired sinusoidal perfusion, and tissue hypoxia elicited in the liver after gut I/R. These KC-mediated responses appear to involve the participation of both PAF and TNF-alpha. (Hepatology 1997 Dec;26(6):1499-505)
- Subjects :
- Pathology
medicine.medical_specialty
Kupffer Cells
Anti-Inflammatory Agents
Ischemia
Gadolinium
Inflammation
medicine.disease_cause
Mice
chemistry.chemical_compound
Fibrinolytic Agents
Internal medicine
Cell Adhesion
Leukocytes
medicine
Animals
Intestinal Mucosa
Hepatology
Platelet-activating factor
Tumor Necrosis Factor-alpha
business.industry
Kupffer cell
Alanine Transaminase
Leukostasis
Azepines
Triazoles
medicine.disease
Intestines
Mice, Inbred C57BL
Chemotaxis, Leukocyte
Oxidative Stress
P-Selectin
medicine.anatomical_structure
Endocrinology
chemistry
Reperfusion Injury
Tumor necrosis factor alpha
Endothelium, Vascular
medicine.symptom
business
NADP
Platelet Aggregation Inhibitors
Intravital microscopy
Oxidative stress
Liver Circulation
Subjects
Details
- ISSN :
- 15273350 and 02709139
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Hepatology
- Accession number :
- edsair.doi.dedup.....7e375df9c700e35d6d8a0d685bf4e77f