Back to Search
Start Over
Recombinant Lactobacillus plantarum NC8 strain expressing porcine rotavirus VP7 induces specific antibodies in BALB/c mice
- Source :
- Acta biochimica et biophysica Sinica. 53(6)
- Publication Year :
- 2020
-
Abstract
- The major etiologic agent that causes acute gastroenteritis worldwide in young animals and children is Group A rotavirus. Currently, commercially available vaccines do not often prevent porcine rotavirus (PRV) infection. In this study, we evaluated the efficacy of oral recombinant Lactobacillus vaccine against PRV in a mouse model. Lactobacillus plantarum NC8 was used as the host strain, and bacterial vectors were constructed, because the NC8 isolated has shown the capability to survive gastric transit and to colonize the intestinal tract of humans and other mammals. To explore the immunological mechanisms, lactic acid bacterial vectors were used to express VP7 antigen from PRV. We constructed an L. plantarum strain with surface-displayed VP7, named NC8-pSIP409-pgsA-VP7-DCpep. The expressed recombinant protein had a molecular weight of ∼37 kDa. The strain was used to immunize BALB/c mice to evaluate their immunomodulatory characteristics. Mice were orally immunized with recombinant L. plantarum NC8-pSIP409-pgsA-VP7-DCpep at a dose of 2 × 109 colony forming units/200 µl. The results showed that NC8-pSIP409-pgsA-VP7-DCpep significantly stimulated the differentiation of dendritic cells (DCs) in Peyer's patches (PPs) and increased the serum levels of IL-4 and IFN-γ, as measured by enzyme-linked immunosorbent assay in mice treated with NC8-pSIP409-pgsA-VP7-DCpep. Compared to the empty vector group, NC8-pSIP409-pgsA-VP7-DCpep significantly increased the production of B220+ B cells in mesenteric lymph nodes (MLNs) and PPs and also increased the titer levels of the VP7-specific antibodies, including IgG and sIgA. The administration of NC8-pSIP409-pgsA-VP7-DCpep mediated relatively broad cellular responses. This study reveals that clear alternatives exist for PRV control strategies and provides information on PRV infection.
- Subjects :
- Rotavirus
Genes, Viral
Swine
viruses
Biophysics
medicine.disease_cause
Antibodies, Viral
Biochemistry
Immunoglobulin G
law.invention
BALB/c
Microbiology
03 medical and health sciences
Mice
fluids and secretions
Immunogenicity, Vaccine
Antigen
law
Lactobacillus
Antigens, Heterophile
medicine
Animals
Antigens, Viral
030304 developmental biology
0303 health sciences
B-Lymphocytes
Mice, Inbred BALB C
Vaccines, Synthetic
biology
030306 microbiology
virus diseases
General Medicine
biology.organism_classification
Recombinant Proteins
Immunoglobulin A, Secretory
Recombinant DNA
biology.protein
Cytokines
Capsid Proteins
Female
Immunization
Antibody
Genetic Engineering
Lactobacillus plantarum
Subjects
Details
- ISSN :
- 17457270
- Volume :
- 53
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Acta biochimica et biophysica Sinica
- Accession number :
- edsair.doi.dedup.....7e335752e9155c74655eadc9fcd184b3