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Complete structure of the core signalling unit of the E. coli chemosensory array in an optimised minicell strain
- Publication Year :
- 2019
- Publisher :
- Cold Spring Harbor Laboratory, 2019.
-
Abstract
- Motile bacteria sense chemical gradients with transmembrane receptors organised in supramolecular signalling arrays.1,2Understanding stimulus detection and transmission at the molecular level requires precise structural characterisation of the array building block known as a core signalling unit (CSU). Here we introduce a novelE. colistrain that forms small minicells possessing extended and highly ordered chemosensory arrays. We provide a three-dimensional (3D) map of a complete CSU at ~16 Å resolution by cryo-electron tomography (cryo-ET) and subtomogram averaging. This map, combined with previously determined high resolution structures and molecular dynamics simulations, yields an atomistic model of the membrane-bound CSU and enables spatial localisation of its signalling domains. Our work thus offers a solid structural basis for interpretation of existing data and design of new experiments to elucidate signalling mechanisms within the CSU and larger array.
- Subjects :
- Physics
0303 health sciences
Motile bacteria
Strain (chemistry)
030302 biochemistry & molecular biology
Resolution (electron density)
Supramolecular chemistry
03 medical and health sciences
Coli strain
Molecular dynamics
Signalling
Core (graph theory)
Biological system
030304 developmental biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7e3181af567cd07a7fd926d40fd0824a
- Full Text :
- https://doi.org/10.1101/723692