Functional evolution of Lepidoptera olfactory receptors revealed by deorphanization of a moth repertoire

Insects detect their hosts or mates primarily through olfaction, and olfactory receptors (ORs) are at the core of odorant detection. Each species has evolved a unique repertoire of ORs whose functional properties are expected to meet its ecological needs, though little is known about the molecular basis of olfaction outside Diptera. Here we report a pioneer functional analysis of a large array of ORs in a lepidopteran, the herbivorous pest Spodoptera littoralis. We demonstrate that most ORs are narrowly tuned to ubiquitous plant volatiles at low, relevant odorant titres. Our phylogenetic analysis highlights a basic conservation of function within the receptor repertoire of Lepidoptera, across the expansive evolutionary radiation of different major clades. Our study provides a reference for further studies of olfactory mechanisms in Lepidoptera, a historically crucial insect order in olfactory research.
The range of odours that an insect can detect depends on its olfactory receptors. Here, the authors functionally characterize the olfactory receptor repertoire of the moth Spodoptera littoralis using the Drosophila empty neuron system and reconstruct the evolution of these receptors in the Lepidoptera.