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Metabolic Plasticity in Cell State Homeostasis and Differentiation of Cultured Human Corneal Endothelial Cells
- Source :
- Investigative ophthalmologyvisual science. 57(10)
- Publication Year :
- 2016
-
Abstract
- Purpose To clarify whether cultured human corneal endothelial cells (cHCECs), heterogeneous in their differentiation state, exhibit distinctive energy metabolism with the aim to develop a reliable method to sort cHCECs applicable for regenerative medicine. Methods The presence of cHCEC subpopulations (SPs) was verified via surface cluster-of-differentiation (CD) marker expression. Cultured HCEC metabolic extracts or corresponding culture supernatants with distinctive cellular phenotypes in regard to energy-metabolism-related functional markers c-Myc and CD44 were prepared and analyzed via capillary electrophoresis-tandem mass spectrometry. The metabolic requirements of heterogeneous SPs of cHCECs were also investigated. Results After successfully discriminating SPs, as verified via surface CD markers in terms of their secretory metabolites, we found that the CD44+++ SP with cell-state transition (CST) exhibited disposition for anaerobic glycolysis, whereas the CD44-SP without CST was disposed to mitochondria-dependent oxidative phosphorylation (OXPHOS). These results raised the possibility of establishing effective culture conditions to selectively expand mature cHCECs with a hexagonal cobblestone shape and inclination for mitochondria-dependent OXPHOS. Conclusions The findings of this study open a pathway for monitoring the disposition of cHCECs via their energy metabolism, thus leading to safe and stable regenerative medicine by use of metabolically defined cHCECs in cell-suspension form.
- Subjects :
- 0301 basic medicine
Adult
Male
Endothelium
Adolescent
Cellular differentiation
Cell Plasticity
Oxidative phosphorylation
Biology
Regenerative medicine
Flow cytometry
Corneal Diseases
03 medical and health sciences
Young Adult
Metabolomics
Tandem Mass Spectrometry
medicine
Homeostasis
Humans
Child
Cells, Cultured
Aged
medicine.diagnostic_test
Endothelium, Corneal
Electrophoresis, Capillary
Cell Differentiation
Middle Aged
Flow Cytometry
030104 developmental biology
medicine.anatomical_structure
Biochemistry
Anaerobic glycolysis
Child, Preschool
Female
Energy Metabolism
Biomarkers
Subjects
Details
- ISSN :
- 15525783
- Volume :
- 57
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Investigative ophthalmologyvisual science
- Accession number :
- edsair.doi.dedup.....7e0ae7723b46e587eaef19954f07ed49