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Impaired interferon‐α expression in plasmacytoid dendritic cells in asthma
- Source :
- Immunity, Inflammation and Disease, Vol 9, Iss 1, Pp 183-195 (2021), Immunity, Inflammation and Disease
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Background Toll‐like receptor (TLR)‐7‐associated rhinovirus (RV) activation is involved in the pathogenesis of asthma. Plasmacytoid dendritic cells (pDCs) are the main interferon‐α‐producing cells against viruses. Objective To determine whether asthmatic patients and control subjects differ in terms of interferon‐α expression in pDCs under TLR‐7 or RV stimulation. Methods pDCs were identified in BDCA‐2+ and HLA‐DR+ peripheral blood mononuclear cells. Interferon‐α expression of pDCs was analyzed after TLR‐7 stimulation with or without interleukin 4 (IL‐4)/IL‐13 pretreatment. Interferon‐α expression was also analyzed after RV stimulation over periods of 24, 48, or 96 h with or without IL‐4 pretreatment. RV detection and molecular typing were assayed from throat swabs. Results Following TLR‐7 stimulation, the expression of intracellular interferon‐α was higher in the pDCs of normal subjects than those of asthmatic patients; however, pretreatment with IL‐4 was shown to reduce this effect. After 48‐ and 96‐h RV stimulation, we observed a notable increase in the production of interferon‐α of pDCs in normal subjects but not in asthmatic patients. Baseline interferon‐α expression in pDCs and the incidence of asthma exacerbation to emergency was higher among the 13% of patients identified as rhinovirus+ than among their RV counterparts. Conclusion Our study discovered the response to TLR‐7 stimulation in pDCs was compromised and the sustainability of interferon‐α expression to RV stimulation was reduced in pDCs of asthmatic patients, which provide further evidence of defective innate response and subspeciality to RV infection in asthma. The high exacerbation history founded in RV+ patients agrees with these findings. Further research is required for the modulatory effect of IL‐4 on TLR‐7 stimulated pDCs.<br />Our study discovered the response to TLR‐7 stimulation in pDCs was compromised and the sustainability of interferon‐a expression to RV stimulation was reduced in pDCs of asthmatic patients, which provide further evidence of defective innate response and subspeciality to RV infection in asthma. The high exacerbation history founded in RV+ patients agrees with these findings.
- Subjects :
- 0301 basic medicine
lcsh:Immunologic diseases. Allergy
Exacerbation
Immunology
Stimulation
medicine.disease_cause
Peripheral blood mononuclear cell
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Interferon
medicine
Humans
Immunology and Allergy
dendritic cells
Receptor
toll‐like receptor 7
Interleukin 4
Original Research
business.industry
Interferon-alpha
hemic and immune systems
asthma
030104 developmental biology
rhinovirus
Toll-Like Receptor 9
Leukocytes, Mononuclear
Rhinovirus
business
lcsh:RC581-607
030215 immunology
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20504527
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Immunity, Inflammation and Disease
- Accession number :
- edsair.doi.dedup.....7e09ecbd18f795515cc9ebe24871df45