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Combination of G-CSF administration and human amniotic fluid mesenchymal stem cell transplantation promotes peripheral nerve regeneration
- Source :
- Neurochemical research. 34(3)
- Publication Year :
- 2008
-
Abstract
- Amniotic fluid mesenchymal stem cells (AFS) harbor the potential to improve peripheral nerve injury by inherited neurotrophic factor secretion, but present the drawback of the short-term survival after transplantation. Granulocyte-colony stimulating factor (G-CSF) has a diversity of functions, including anti-inflammatory and anti-apoptotic effects. This study was conducted to evaluate whether G-CSF could augment the neuroprotective effect of transplanted AFS against peripheral nerve injury. The potential involvement of anti-inflammation/anti-apoptosis effect was also investigated. Peripheral nerve injury was produced in Sprauge-Dawley rats by crushing left sciatic nerve using a vessel clamp. The AFS were embedded in fibrin glue and delivered to the injured site. G-CSF (50 microg/kg) was administrated by intra-peritoneal injection for 7 consecutive days. Cell apoptosis, inflammatory cytokines, motor function, and nerve regeneration were evaluated 7 or 28 days after injury. Crush injury induced inflammatory response, disrupted nerve integrity, and impaired nerve function in sciatic nerve. Crush injury-provoked inflammation was attenuated in groups receiving G-CSF but not in AFS only group. In transplanted AFS, marked apoptosis was detected and this event was reduced by G-CSF treatment. Increased nerve myelination and improved motor function were observed in AFS transplanted, G-CSF administrated, and AFS/G-CSF combined treatment groups. Significantly, the combined treatment showed the most beneficial effect. In conclusion, the concomitant treatment of AFS with G-CSF augments peripheral nerve regeneration which may involve the suppression of apoptotic death in implanted AFS and the attenuation of inflammatory response.
- Subjects :
- Pathology
medicine.medical_specialty
Time Factors
Nerve Crush
Anti-Inflammatory Agents
Neural Conduction
Apoptosis
Mesenchymal Stem Cell Transplantation
Biochemistry
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
Neurotrophic factors
Granulocyte Colony-Stimulating Factor
medicine
Animals
Humans
Spinal cord injury
Inflammation
business.industry
Mesenchymal stem cell
General Medicine
Sciatic nerve injury
medicine.disease
Amniotic Fluid
Sciatic Nerve
Nerve Regeneration
Rats
Transplantation
Neuroprotective Agents
Immunology
Peripheral nerve injury
Crush injury
Cytokines
Sciatic nerve
business
Subjects
Details
- ISSN :
- 15736903
- Volume :
- 34
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Neurochemical research
- Accession number :
- edsair.doi.dedup.....7dff5b3aebbbea7fdd6f32dea9686c0b